Avaliação do reparo tecidual em defeitos críticos enxertados com osso autógeno e membrana de celulose bacteriana derivatizada com alendronato: análise bioquímica e microtomográfica

• Ano de defesa: 2019
• Autor(a) principal: Nascimento, Tuanny Carvalho de Lima do
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Odontologia Clínica; UP
• Palavras-chave em Português: Odontologia; Alendronato; Celulose; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2090

The objective of this study is the analysis of the bacterial cellulose membrane derived with alendronate in bone repair. Sixty-one rats were divided into 5 groups, the control group being: bovine bone cortical reabsorbable membrane; Group 1: bacterial cellulose membrane in natura, Group 2: bacterial cellulose membrane - mercerized - derivatized with alendronate; Group 3: bacterial cellulose membrane -mercerized and negatively charged - derivatized with alendronate, and Group 4: bacterial cellulose membrane - mercerized and positively charged - derivatized with alendronate. In all animals the only critical function was 5 mm in calvaria, and the bone was particulate and grafted again in another position and covered by membrane. Cardiological puncture was performed to evaluate the five groups in the preoperative period and euthanasia. After 15 or 60 days, the genes were euthanized. The collected plasma was submitted to biochemistry to evaluate the biomarkers of osteoprotegerin (OPG), parathormone (PTH), sclerostin (SOST) and fibroblast growth factor 23 (FGF23), by means of the technology. The removed ducts were submitted to microtomographic analysis to evaluate parameters related to quantitative and qualitative bone repair. The results were submitted to statistical analysis, Wilcoxon test and Kruskal Wallis test, with significance level of 0.05. No differences were found between the biomarkers between groups (p > 0.05). It is also not a relationship between the same biomarker at different times (p > 0.05). In the quantitative microtomographic analysis it was observed a higher percentage of bone formed in the control group of 15 days, group 1 and group 2 of 60 days. Also, in group 1 of 60 days it showed in the trabecular a greater number of trabeculae and less space between one trabecula and another. Thus, results of the results, it is concluded that the bacterial cellulose membrane in natura results promising results that do not refer to a barrier favorable to bone repair.