Avaliação do reparo tecidual em defeitos críticos enxertados com osso autógeno e membranas de celulose bacteriana ionizadas e derivatizadas com alendronato

• Ano de defesa: 2019
• Autor(a) principal: Verbicaro, Thalyta
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Odontologia Clínica; UP
• Palavras-chave em Português: Odontologia clínica; Celulose; Osteocalcina; Alendronato; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2159

Introduction: biomaterials used in the bone repair process must present well-known physicochemical properties and a tested biocompatibility to determine if there is satisfactory interaction between host and material used. Cellulose is used in tissue engineering because its good mechanical properties and chemical structure that allows the incorporation of other substances. Objective: evaluate in vivo biocompatibility of derivatized vegetable and bacterial cellulose membranes with alendronate. Methodological procedures: eighty-four rats were randomly divided into 7 groups, named: in natura bacterial cellulose (in natura CB), mercerized bacterial cellulose (mercerized CB), anionic bacterial cellulose with alendronate (anionic CB), cationic bacterial cellulose with alendronate (CB), vegetable cellulose (CV), 5% vegetable cellulose with alendronate (CV 5%), and 1% vegetable cellulose with alendronate (CV 1%). After sedation and anesthesia, a 1.0 cm incision was made in the back, exposing the subcutaneous tissue, and a membrane was implanted in the region. The suture was performed with simple stitches. The experimental timeframes were 15 and 60 days. Histological slides were made, analyzed and the parameters evaluated were acute inflammatory process intensity, presence of plasmocytes and macrophages, necrosis, fibrosis, vascularization and presence of membrane. Results: Regarding the intensity of the acute inflammatory process, it was possible to observe that the mercerized CB and cationic CB membranes presented a higher severe inflammatory process in relation to CV (p <0.05) within 15 days. At 60 days, the mercerized CB membrane presented a higher acute inflammatory process in relation to the CV, 5% CV and 1% CV membranes (p <0.05). When necrosis was analyzed at 60 days, the mercerized CB group presented in all operated animals. Conclusion: Mercerized CB membrane presented acute inflammatory process at 15 and 60 days, and signs of tissue necrosis in all animals operated in the longest experimental time. In 60 days, the cellulose membranes presented multinucleated giant cells with granulomas formation. Therefore, these membranes showed unsatisfactory biological response and were not considered biocompatible.