Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE

Bibliographic Details
Main Author: Portela, Pilar C.
Publication Date: 2024
Other Authors: Shipps, Catharine C., Shen, Cong, Srikanth, Vishok, Salgueiro, Carlos A., Malvankar, Nikhil S.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/179275
Summary: We thank Gary Brudvig for advice on spectroelectrochemistry measurements and M. Gubermann-Pfeffer for help with ZDOCK. This research was supported by the National Defense Science and Engineering Graduate Fellowship (to C.C.S.), the Human Frontier Science Program award no. RGP017/2023 (to N.S.M. and C.A. S.), NSF CAREER award no. 1749662 (to N.S.M.), the NSF-ANR award no. 2210473 (to N.S.M.), and the National Institutes of Health (NIH) Director’s New Innovator award (1DP2AI138259-01 to N.S.M.). Publisher Copyright: © The Author(s) 2024
id RCAP_ce062ece9e3b4296e87d339b1952e534
oai_identifier_str oai:run.unl.pt:10362/179275
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDEChemistry(all)Biochemistry, Genetics and Molecular Biology(all)Physics and Astronomy(all)SDG 7 - Affordable and Clean EnergySDG 15 - Life on LandWe thank Gary Brudvig for advice on spectroelectrochemistry measurements and M. Gubermann-Pfeffer for help with ZDOCK. This research was supported by the National Defense Science and Engineering Graduate Fellowship (to C.C.S.), the Human Frontier Science Program award no. RGP017/2023 (to N.S.M. and C.A. S.), NSF CAREER award no. 1749662 (to N.S.M.), the NSF-ANR award no. 2210473 (to N.S.M.), and the National Institutes of Health (NIH) Director’s New Innovator award (1DP2AI138259-01 to N.S.M.). Publisher Copyright: © The Author(s) 2024Extracellular electron transfer (EET) via microbial nanowires drives globally-important environmental processes and biotechnological applications for bioenergy, bioremediation, and bioelectronics. Due to highly-redundant and complex EET pathways, it is unclear how microbes wire electrons rapidly (>106s−1) from the inner-membrane through outer-surface nanowires directly to an external environment despite a crowded periplasm and slow (<105s−1) electron diffusion among periplasmic cytochromes. Here, we show that Geobacter sulfurreducens periplasmic cytochromes PpcABCDE inject electrons directly into OmcS nanowires by binding transiently with differing efficiencies, with the least-abundant cytochrome (PpcC) showing the highest efficiency. Remarkably, this defined nanowire-charging pathway is evolutionarily conserved in phylogenetically-diverse bacteria capable of EET. OmcS heme reduction potentials are within 200 mV of each other, with a midpoint 82 mV-higher than reported previously. This could explain efficient EET over micrometres at ultrafast (<200 fs) rates with negligible energy loss. Engineering this minimal nanowire-charging pathway may yield microbial chassis with improved performance.Faculdade de Ciências e Tecnologia (FCT)UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNPortela, Pilar C.Shipps, Catharine C.Shen, CongSrikanth, VishokSalgueiro, Carlos A.Malvankar, Nikhil S.2025-02-18T21:19:45Z2024-122024-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/179275eng2041-1723PURE: 106515667https://doi.org/10.1038/s41467-024-46192-0info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-02-24T01:48:54Zoai:run.unl.pt:10362/179275Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T20:40:11.596514Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE
title Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE
spellingShingle Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE
Portela, Pilar C.
Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
SDG 7 - Affordable and Clean Energy
SDG 15 - Life on Land
title_short Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE
title_full Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE
title_fullStr Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE
title_full_unstemmed Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE
title_sort Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE
author Portela, Pilar C.
author_facet Portela, Pilar C.
Shipps, Catharine C.
Shen, Cong
Srikanth, Vishok
Salgueiro, Carlos A.
Malvankar, Nikhil S.
author_role author
author2 Shipps, Catharine C.
Shen, Cong
Srikanth, Vishok
Salgueiro, Carlos A.
Malvankar, Nikhil S.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Faculdade de Ciências e Tecnologia (FCT)
UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Portela, Pilar C.
Shipps, Catharine C.
Shen, Cong
Srikanth, Vishok
Salgueiro, Carlos A.
Malvankar, Nikhil S.
dc.subject.por.fl_str_mv Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
SDG 7 - Affordable and Clean Energy
SDG 15 - Life on Land
topic Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
SDG 7 - Affordable and Clean Energy
SDG 15 - Life on Land
description We thank Gary Brudvig for advice on spectroelectrochemistry measurements and M. Gubermann-Pfeffer for help with ZDOCK. This research was supported by the National Defense Science and Engineering Graduate Fellowship (to C.C.S.), the Human Frontier Science Program award no. RGP017/2023 (to N.S.M. and C.A. S.), NSF CAREER award no. 1749662 (to N.S.M.), the NSF-ANR award no. 2210473 (to N.S.M.), and the National Institutes of Health (NIH) Director’s New Innovator award (1DP2AI138259-01 to N.S.M.). Publisher Copyright: © The Author(s) 2024
publishDate 2024
dc.date.none.fl_str_mv 2024-12
2024-12-01T00:00:00Z
2025-02-18T21:19:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/179275
url http://hdl.handle.net/10362/179275
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2041-1723
PURE: 106515667
https://doi.org/10.1038/s41467-024-46192-0
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833598785201111040

Similar Items