An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation
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Publication Date: | 2018 |
Other Authors: | , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
Download full: | https://doi.org/10.1038/s41467-018-07839-x |
Summary: | The authors would like to thank Manuela M. Pereira for helpful discussions on liposome experiments and for provision of the ActC structure prior to publication, and Miguel Teixeira for use of the dual-wavelength OLIS spectrophotometer. This work was financially supported by Fundacao para a Ciencia e Tecnologia (Portugal) through fellowships SFRH/BPD/84607/2012 (to A.G.D.) and SFRH/BPD/92537/2013 (to D.L.), grants PTDC/BIA-MIC/6512/2014 and PTDC/BIA-MIC/2723/2014 (to I.A.C.P.) and R&D units UID/Multi/04551/2013 (Green-IT) and LISBOA-01-0145-FEDER-007660 (MostMicro) cofunded by FCT/MCTES and FEDER funds through COMPETE2020/POCI. Funding by the WWTF (VRG15-007 to F.L.S.), BBSRC UK (Grant K00929X to T.A.C.), and the European Union's Horizon 2020 research and innovation programme (Grant agreement no. 810856) is also acknowledged. |
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An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservationChemistry(all)Biochemistry, Genetics and Molecular Biology(all)Physics and Astronomy(all)SDG 7 - Affordable and Clean EnergyThe authors would like to thank Manuela M. Pereira for helpful discussions on liposome experiments and for provision of the ActC structure prior to publication, and Miguel Teixeira for use of the dual-wavelength OLIS spectrophotometer. This work was financially supported by Fundacao para a Ciencia e Tecnologia (Portugal) through fellowships SFRH/BPD/84607/2012 (to A.G.D.) and SFRH/BPD/92537/2013 (to D.L.), grants PTDC/BIA-MIC/6512/2014 and PTDC/BIA-MIC/2723/2014 (to I.A.C.P.) and R&D units UID/Multi/04551/2013 (Green-IT) and LISBOA-01-0145-FEDER-007660 (MostMicro) cofunded by FCT/MCTES and FEDER funds through COMPETE2020/POCI. Funding by the WWTF (VRG15-007 to F.L.S.), BBSRC UK (Grant K00929X to T.A.C.), and the European Union's Horizon 2020 research and innovation programme (Grant agreement no. 810856) is also acknowledged.The bioenergetics of anaerobic metabolism frequently relies on redox loops performed by membrane complexes with substrate- and quinone-binding sites on opposite sides of the membrane. However, in sulfate respiration (a key process in the biogeochemical sulfur cycle), the substrate- and quinone-binding sites of the QrcABCD complex are periplasmic, and their role in energy conservation has not been elucidated. Here we show that the QrcABCD complex of Desulfovibrio vulgaris is electrogenic, as protons and electrons required for quinone reduction are extracted from opposite sides of the membrane, with a H+/e− ratio of 1. Although the complex does not act as a H+-pump, QrcD may include a conserved proton channel leading from the N-side to the P-side menaquinone pocket. Our work provides evidence of how energy is conserved during dissimilatory sulfate reduction, and suggests mechanisms behind the functions of related bacterial respiratory complexes in other bioenergetic contexts.Molecular, Structural and Cellular Microbiology (MOSTMICRO)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)DQ - Departamento de QuímicaBioresources 4 Sustainability (GREEN-IT)RUNDuarte, Américo G.Catarino, TeresaWhite, Gaye F.Lousa, DianaNeukirchen, SinjeSoares, Cláudio M.Sousa, Filipa L.Clarke, Thomas A.Pereira, Inês A. C.2019-04-29T22:15:56Z2018-12-012018-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1038/s41467-018-07839-xeng2041-1723PURE: 11734313http://www.scopus.com/inward/record.url?scp=85058913300&partnerID=8YFLogxKhttps://doi.org/10.1038/s41467-018-07839-xinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:39:03Zoai:run.unl.pt:10362/68103Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:10:05.376131Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
dc.title.none.fl_str_mv |
An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation |
title |
An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation |
spellingShingle |
An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation Duarte, Américo G. Chemistry(all) Biochemistry, Genetics and Molecular Biology(all) Physics and Astronomy(all) SDG 7 - Affordable and Clean Energy |
title_short |
An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation |
title_full |
An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation |
title_fullStr |
An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation |
title_full_unstemmed |
An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation |
title_sort |
An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation |
author |
Duarte, Américo G. |
author_facet |
Duarte, Américo G. Catarino, Teresa White, Gaye F. Lousa, Diana Neukirchen, Sinje Soares, Cláudio M. Sousa, Filipa L. Clarke, Thomas A. Pereira, Inês A. C. |
author_role |
author |
author2 |
Catarino, Teresa White, Gaye F. Lousa, Diana Neukirchen, Sinje Soares, Cláudio M. Sousa, Filipa L. Clarke, Thomas A. Pereira, Inês A. C. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Molecular, Structural and Cellular Microbiology (MOSTMICRO) Instituto de Tecnologia Química e Biológica António Xavier (ITQB) DQ - Departamento de Química Bioresources 4 Sustainability (GREEN-IT) RUN |
dc.contributor.author.fl_str_mv |
Duarte, Américo G. Catarino, Teresa White, Gaye F. Lousa, Diana Neukirchen, Sinje Soares, Cláudio M. Sousa, Filipa L. Clarke, Thomas A. Pereira, Inês A. C. |
dc.subject.por.fl_str_mv |
Chemistry(all) Biochemistry, Genetics and Molecular Biology(all) Physics and Astronomy(all) SDG 7 - Affordable and Clean Energy |
topic |
Chemistry(all) Biochemistry, Genetics and Molecular Biology(all) Physics and Astronomy(all) SDG 7 - Affordable and Clean Energy |
description |
The authors would like to thank Manuela M. Pereira for helpful discussions on liposome experiments and for provision of the ActC structure prior to publication, and Miguel Teixeira for use of the dual-wavelength OLIS spectrophotometer. This work was financially supported by Fundacao para a Ciencia e Tecnologia (Portugal) through fellowships SFRH/BPD/84607/2012 (to A.G.D.) and SFRH/BPD/92537/2013 (to D.L.), grants PTDC/BIA-MIC/6512/2014 and PTDC/BIA-MIC/2723/2014 (to I.A.C.P.) and R&D units UID/Multi/04551/2013 (Green-IT) and LISBOA-01-0145-FEDER-007660 (MostMicro) cofunded by FCT/MCTES and FEDER funds through COMPETE2020/POCI. Funding by the WWTF (VRG15-007 to F.L.S.), BBSRC UK (Grant K00929X to T.A.C.), and the European Union's Horizon 2020 research and innovation programme (Grant agreement no. 810856) is also acknowledged. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 2018-12-01T00:00:00Z 2019-04-29T22:15:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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dc.identifier.uri.fl_str_mv |
https://doi.org/10.1038/s41467-018-07839-x |
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https://doi.org/10.1038/s41467-018-07839-x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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2041-1723 PURE: 11734313 http://www.scopus.com/inward/record.url?scp=85058913300&partnerID=8YFLogxK https://doi.org/10.1038/s41467-018-07839-x |
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