An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation

Bibliographic Details
Main Author: Duarte, Américo G.
Publication Date: 2018
Other Authors: Catarino, Teresa, White, Gaye F., Lousa, Diana, Neukirchen, Sinje, Soares, Cláudio M., Sousa, Filipa L., Clarke, Thomas A., Pereira, Inês A. C.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://doi.org/10.1038/s41467-018-07839-x
Summary: The authors would like to thank Manuela M. Pereira for helpful discussions on liposome experiments and for provision of the ActC structure prior to publication, and Miguel Teixeira for use of the dual-wavelength OLIS spectrophotometer. This work was financially supported by Fundacao para a Ciencia e Tecnologia (Portugal) through fellowships SFRH/BPD/84607/2012 (to A.G.D.) and SFRH/BPD/92537/2013 (to D.L.), grants PTDC/BIA-MIC/6512/2014 and PTDC/BIA-MIC/2723/2014 (to I.A.C.P.) and R&D units UID/Multi/04551/2013 (Green-IT) and LISBOA-01-0145-FEDER-007660 (MostMicro) cofunded by FCT/MCTES and FEDER funds through COMPETE2020/POCI. Funding by the WWTF (VRG15-007 to F.L.S.), BBSRC UK (Grant K00929X to T.A.C.), and the European Union's Horizon 2020 research and innovation programme (Grant agreement no. 810856) is also acknowledged.
id RCAP_439fd3c4065316871c38c98e096db41a
oai_identifier_str oai:run.unl.pt:10362/68103
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservationChemistry(all)Biochemistry, Genetics and Molecular Biology(all)Physics and Astronomy(all)SDG 7 - Affordable and Clean EnergyThe authors would like to thank Manuela M. Pereira for helpful discussions on liposome experiments and for provision of the ActC structure prior to publication, and Miguel Teixeira for use of the dual-wavelength OLIS spectrophotometer. This work was financially supported by Fundacao para a Ciencia e Tecnologia (Portugal) through fellowships SFRH/BPD/84607/2012 (to A.G.D.) and SFRH/BPD/92537/2013 (to D.L.), grants PTDC/BIA-MIC/6512/2014 and PTDC/BIA-MIC/2723/2014 (to I.A.C.P.) and R&D units UID/Multi/04551/2013 (Green-IT) and LISBOA-01-0145-FEDER-007660 (MostMicro) cofunded by FCT/MCTES and FEDER funds through COMPETE2020/POCI. Funding by the WWTF (VRG15-007 to F.L.S.), BBSRC UK (Grant K00929X to T.A.C.), and the European Union's Horizon 2020 research and innovation programme (Grant agreement no. 810856) is also acknowledged.The bioenergetics of anaerobic metabolism frequently relies on redox loops performed by membrane complexes with substrate- and quinone-binding sites on opposite sides of the membrane. However, in sulfate respiration (a key process in the biogeochemical sulfur cycle), the substrate- and quinone-binding sites of the QrcABCD complex are periplasmic, and their role in energy conservation has not been elucidated. Here we show that the QrcABCD complex of Desulfovibrio vulgaris is electrogenic, as protons and electrons required for quinone reduction are extracted from opposite sides of the membrane, with a H+/e− ratio of 1. Although the complex does not act as a H+-pump, QrcD may include a conserved proton channel leading from the N-side to the P-side menaquinone pocket. Our work provides evidence of how energy is conserved during dissimilatory sulfate reduction, and suggests mechanisms behind the functions of related bacterial respiratory complexes in other bioenergetic contexts.Molecular, Structural and Cellular Microbiology (MOSTMICRO)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)DQ - Departamento de QuímicaBioresources 4 Sustainability (GREEN-IT)RUNDuarte, Américo G.Catarino, TeresaWhite, Gaye F.Lousa, DianaNeukirchen, SinjeSoares, Cláudio M.Sousa, Filipa L.Clarke, Thomas A.Pereira, Inês A. C.2019-04-29T22:15:56Z2018-12-012018-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1038/s41467-018-07839-xeng2041-1723PURE: 11734313http://www.scopus.com/inward/record.url?scp=85058913300&partnerID=8YFLogxKhttps://doi.org/10.1038/s41467-018-07839-xinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:39:03Zoai:run.unl.pt:10362/68103Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:10:05.376131Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation
title An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation
spellingShingle An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation
Duarte, Américo G.
Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
SDG 7 - Affordable and Clean Energy
title_short An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation
title_full An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation
title_fullStr An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation
title_full_unstemmed An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation
title_sort An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation
author Duarte, Américo G.
author_facet Duarte, Américo G.
Catarino, Teresa
White, Gaye F.
Lousa, Diana
Neukirchen, Sinje
Soares, Cláudio M.
Sousa, Filipa L.
Clarke, Thomas A.
Pereira, Inês A. C.
author_role author
author2 Catarino, Teresa
White, Gaye F.
Lousa, Diana
Neukirchen, Sinje
Soares, Cláudio M.
Sousa, Filipa L.
Clarke, Thomas A.
Pereira, Inês A. C.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Molecular, Structural and Cellular Microbiology (MOSTMICRO)
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
DQ - Departamento de Química
Bioresources 4 Sustainability (GREEN-IT)
RUN
dc.contributor.author.fl_str_mv Duarte, Américo G.
Catarino, Teresa
White, Gaye F.
Lousa, Diana
Neukirchen, Sinje
Soares, Cláudio M.
Sousa, Filipa L.
Clarke, Thomas A.
Pereira, Inês A. C.
dc.subject.por.fl_str_mv Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
SDG 7 - Affordable and Clean Energy
topic Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
SDG 7 - Affordable and Clean Energy
description The authors would like to thank Manuela M. Pereira for helpful discussions on liposome experiments and for provision of the ActC structure prior to publication, and Miguel Teixeira for use of the dual-wavelength OLIS spectrophotometer. This work was financially supported by Fundacao para a Ciencia e Tecnologia (Portugal) through fellowships SFRH/BPD/84607/2012 (to A.G.D.) and SFRH/BPD/92537/2013 (to D.L.), grants PTDC/BIA-MIC/6512/2014 and PTDC/BIA-MIC/2723/2014 (to I.A.C.P.) and R&D units UID/Multi/04551/2013 (Green-IT) and LISBOA-01-0145-FEDER-007660 (MostMicro) cofunded by FCT/MCTES and FEDER funds through COMPETE2020/POCI. Funding by the WWTF (VRG15-007 to F.L.S.), BBSRC UK (Grant K00929X to T.A.C.), and the European Union's Horizon 2020 research and innovation programme (Grant agreement no. 810856) is also acknowledged.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-01
2018-12-01T00:00:00Z
2019-04-29T22:15:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1038/s41467-018-07839-x
url https://doi.org/10.1038/s41467-018-07839-x
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2041-1723
PURE: 11734313
http://www.scopus.com/inward/record.url?scp=85058913300&partnerID=8YFLogxK
https://doi.org/10.1038/s41467-018-07839-x
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833596484191256576