Structure of a membrane-bound menaquinol:organohalide oxidoreductase
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Publication Date: | 2023 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
Download full: | http://hdl.handle.net/10362/165734 |
Summary: | Funding Information: We thank the Dubochet Center for Imaging (DCI) in Lausanne for cryo-EM data collection. DCI is an initiative of the EPFL, University of Lausanne and University of Geneva. We also thank Kelvin Lau from PTPSP laboratory (EPFL) and the PCF laboratory (EPFL). This work was supported by the Swiss National Science Foundation (grant N° CRSII5_177195 and grant N° 310030_188548 to HS; grant N° 31003 A 173059 to CH), the Sino-Swiss Science and Technology Cooperation SSSTC (N° IZLCZ0_206089 to HS), the Fundação para a Ciência e Tecnologia for support through the MOSTMICRO-ITQB unit (UIDB/04612/2020 and UIDP/04612/2020 to AGD and IACP) and the Associated Laboratory LS4FUTURE (LA/P/0087/2020 to AGD and IACP). Funding Information: We thank the Dubochet Center for Imaging (DCI) in Lausanne for cryo-EM data collection. DCI is an initiative of the EPFL, University of Lausanne and University of Geneva. We also thank Kelvin Lau from PTPSP laboratory (EPFL) and the PCF laboratory (EPFL). This work was supported by the Swiss National Science Foundation (grant N° CRSII5_177195 and grant N° 310030_188548 to HS; grant N° 31003 A 173059 to CH), the Sino-Swiss Science and Technology Cooperation SSSTC (N° IZLCZ0_206089 to HS), the Fundação para a Ciência e Tecnologia for support through the MOSTMICRO-ITQB unit (UIDB/04612/2020 and UIDP/04612/2020 to AGD and IACP) and the Associated Laboratory LS4FUTURE (LA/P/0087/2020 to AGD and IACP). Publisher Copyright: © 2023, The Author(s). |
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Structure of a membrane-bound menaquinol:organohalide oxidoreductaseChemistry(all)Biochemistry, Genetics and Molecular Biology(all)Physics and Astronomy(all)SDG 7 - Affordable and Clean EnergyFunding Information: We thank the Dubochet Center for Imaging (DCI) in Lausanne for cryo-EM data collection. DCI is an initiative of the EPFL, University of Lausanne and University of Geneva. We also thank Kelvin Lau from PTPSP laboratory (EPFL) and the PCF laboratory (EPFL). This work was supported by the Swiss National Science Foundation (grant N° CRSII5_177195 and grant N° 310030_188548 to HS; grant N° 31003 A 173059 to CH), the Sino-Swiss Science and Technology Cooperation SSSTC (N° IZLCZ0_206089 to HS), the Fundação para a Ciência e Tecnologia for support through the MOSTMICRO-ITQB unit (UIDB/04612/2020 and UIDP/04612/2020 to AGD and IACP) and the Associated Laboratory LS4FUTURE (LA/P/0087/2020 to AGD and IACP). Funding Information: We thank the Dubochet Center for Imaging (DCI) in Lausanne for cryo-EM data collection. DCI is an initiative of the EPFL, University of Lausanne and University of Geneva. We also thank Kelvin Lau from PTPSP laboratory (EPFL) and the PCF laboratory (EPFL). This work was supported by the Swiss National Science Foundation (grant N° CRSII5_177195 and grant N° 310030_188548 to HS; grant N° 31003 A 173059 to CH), the Sino-Swiss Science and Technology Cooperation SSSTC (N° IZLCZ0_206089 to HS), the Fundação para a Ciência e Tecnologia for support through the MOSTMICRO-ITQB unit (UIDB/04612/2020 and UIDP/04612/2020 to AGD and IACP) and the Associated Laboratory LS4FUTURE (LA/P/0087/2020 to AGD and IACP). Publisher Copyright: © 2023, The Author(s).Organohalide-respiring bacteria are key organisms for the bioremediation of soils and aquifers contaminated with halogenated organic compounds. The major players in this process are respiratory reductive dehalogenases, corrinoid enzymes that use organohalides as substrates and contribute to energy conservation. Here, we present the structure of a menaquinol:organohalide oxidoreductase obtained by cryo-EM. The membrane-bound protein was isolated from Desulfitobacterium hafniense strain TCE1 as a PceA2B2 complex catalysing the dechlorination of tetrachloroethene. Two catalytic PceA subunits are anchored to the membrane by two small integral membrane PceB subunits. The structure reveals two menaquinone molecules bound at the interface of the two different subunits, which are the starting point of a chain of redox cofactors for electron transfer to the active site. In this work, the structure elucidates how energy is conserved during organohalide respiration in menaquinone-dependent organohalide-respiring bacteria.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNCimmino, LorenzoDuarte, Américo G.Ni, DongchunEkundayo, Babatunde E.Pereira, Inês A.C.Stahlberg, HenningHolliger, ChristofMaillard, Julien2024-04-02T23:47:43Z2023-122023-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/165734eng2041-1723PURE: 83672405https://doi.org/10.1038/s41467-023-42927-7info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:20:08Zoai:run.unl.pt:10362/165734Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:50:52.954240Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
dc.title.none.fl_str_mv |
Structure of a membrane-bound menaquinol:organohalide oxidoreductase |
title |
Structure of a membrane-bound menaquinol:organohalide oxidoreductase |
spellingShingle |
Structure of a membrane-bound menaquinol:organohalide oxidoreductase Cimmino, Lorenzo Chemistry(all) Biochemistry, Genetics and Molecular Biology(all) Physics and Astronomy(all) SDG 7 - Affordable and Clean Energy |
title_short |
Structure of a membrane-bound menaquinol:organohalide oxidoreductase |
title_full |
Structure of a membrane-bound menaquinol:organohalide oxidoreductase |
title_fullStr |
Structure of a membrane-bound menaquinol:organohalide oxidoreductase |
title_full_unstemmed |
Structure of a membrane-bound menaquinol:organohalide oxidoreductase |
title_sort |
Structure of a membrane-bound menaquinol:organohalide oxidoreductase |
author |
Cimmino, Lorenzo |
author_facet |
Cimmino, Lorenzo Duarte, Américo G. Ni, Dongchun Ekundayo, Babatunde E. Pereira, Inês A.C. Stahlberg, Henning Holliger, Christof Maillard, Julien |
author_role |
author |
author2 |
Duarte, Américo G. Ni, Dongchun Ekundayo, Babatunde E. Pereira, Inês A.C. Stahlberg, Henning Holliger, Christof Maillard, Julien |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
dc.contributor.author.fl_str_mv |
Cimmino, Lorenzo Duarte, Américo G. Ni, Dongchun Ekundayo, Babatunde E. Pereira, Inês A.C. Stahlberg, Henning Holliger, Christof Maillard, Julien |
dc.subject.por.fl_str_mv |
Chemistry(all) Biochemistry, Genetics and Molecular Biology(all) Physics and Astronomy(all) SDG 7 - Affordable and Clean Energy |
topic |
Chemistry(all) Biochemistry, Genetics and Molecular Biology(all) Physics and Astronomy(all) SDG 7 - Affordable and Clean Energy |
description |
Funding Information: We thank the Dubochet Center for Imaging (DCI) in Lausanne for cryo-EM data collection. DCI is an initiative of the EPFL, University of Lausanne and University of Geneva. We also thank Kelvin Lau from PTPSP laboratory (EPFL) and the PCF laboratory (EPFL). This work was supported by the Swiss National Science Foundation (grant N° CRSII5_177195 and grant N° 310030_188548 to HS; grant N° 31003 A 173059 to CH), the Sino-Swiss Science and Technology Cooperation SSSTC (N° IZLCZ0_206089 to HS), the Fundação para a Ciência e Tecnologia for support through the MOSTMICRO-ITQB unit (UIDB/04612/2020 and UIDP/04612/2020 to AGD and IACP) and the Associated Laboratory LS4FUTURE (LA/P/0087/2020 to AGD and IACP). Funding Information: We thank the Dubochet Center for Imaging (DCI) in Lausanne for cryo-EM data collection. DCI is an initiative of the EPFL, University of Lausanne and University of Geneva. We also thank Kelvin Lau from PTPSP laboratory (EPFL) and the PCF laboratory (EPFL). This work was supported by the Swiss National Science Foundation (grant N° CRSII5_177195 and grant N° 310030_188548 to HS; grant N° 31003 A 173059 to CH), the Sino-Swiss Science and Technology Cooperation SSSTC (N° IZLCZ0_206089 to HS), the Fundação para a Ciência e Tecnologia for support through the MOSTMICRO-ITQB unit (UIDB/04612/2020 and UIDP/04612/2020 to AGD and IACP) and the Associated Laboratory LS4FUTURE (LA/P/0087/2020 to AGD and IACP). Publisher Copyright: © 2023, The Author(s). |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12 2023-12-01T00:00:00Z 2024-04-02T23:47:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/165734 |
url |
http://hdl.handle.net/10362/165734 |
dc.language.iso.fl_str_mv |
eng |
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2041-1723 PURE: 83672405 https://doi.org/10.1038/s41467-023-42927-7 |
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