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Structure of a membrane-bound menaquinol:organohalide oxidoreductase

Detalhes bibliográficos
Autor(a) principal: Cimmino, Lorenzo
Data de Publicação: 2023
Outros Autores: Duarte, Américo G., Ni, Dongchun, Ekundayo, Babatunde E., Pereira, Inês A.C., Stahlberg, Henning, Holliger, Christof, Maillard, Julien
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10362/165734
Resumo: Funding Information: We thank the Dubochet Center for Imaging (DCI) in Lausanne for cryo-EM data collection. DCI is an initiative of the EPFL, University of Lausanne and University of Geneva. We also thank Kelvin Lau from PTPSP laboratory (EPFL) and the PCF laboratory (EPFL). This work was supported by the Swiss National Science Foundation (grant N° CRSII5_177195 and grant N° 310030_188548 to HS; grant N° 31003 A 173059 to CH), the Sino-Swiss Science and Technology Cooperation SSSTC (N° IZLCZ0_206089 to HS), the Fundação para a Ciência e Tecnologia for support through the MOSTMICRO-ITQB unit (UIDB/04612/2020 and UIDP/04612/2020 to AGD and IACP) and the Associated Laboratory LS4FUTURE (LA/P/0087/2020 to AGD and IACP). Funding Information: We thank the Dubochet Center for Imaging (DCI) in Lausanne for cryo-EM data collection. DCI is an initiative of the EPFL, University of Lausanne and University of Geneva. We also thank Kelvin Lau from PTPSP laboratory (EPFL) and the PCF laboratory (EPFL). This work was supported by the Swiss National Science Foundation (grant N° CRSII5_177195 and grant N° 310030_188548 to HS; grant N° 31003 A 173059 to CH), the Sino-Swiss Science and Technology Cooperation SSSTC (N° IZLCZ0_206089 to HS), the Fundação para a Ciência e Tecnologia for support through the MOSTMICRO-ITQB unit (UIDB/04612/2020 and UIDP/04612/2020 to AGD and IACP) and the Associated Laboratory LS4FUTURE (LA/P/0087/2020 to AGD and IACP). Publisher Copyright: © 2023, The Author(s).
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spelling Structure of a membrane-bound menaquinol:organohalide oxidoreductaseChemistry(all)Biochemistry, Genetics and Molecular Biology(all)Physics and Astronomy(all)SDG 7 - Affordable and Clean EnergyFunding Information: We thank the Dubochet Center for Imaging (DCI) in Lausanne for cryo-EM data collection. DCI is an initiative of the EPFL, University of Lausanne and University of Geneva. We also thank Kelvin Lau from PTPSP laboratory (EPFL) and the PCF laboratory (EPFL). This work was supported by the Swiss National Science Foundation (grant N° CRSII5_177195 and grant N° 310030_188548 to HS; grant N° 31003 A 173059 to CH), the Sino-Swiss Science and Technology Cooperation SSSTC (N° IZLCZ0_206089 to HS), the Fundação para a Ciência e Tecnologia for support through the MOSTMICRO-ITQB unit (UIDB/04612/2020 and UIDP/04612/2020 to AGD and IACP) and the Associated Laboratory LS4FUTURE (LA/P/0087/2020 to AGD and IACP). Funding Information: We thank the Dubochet Center for Imaging (DCI) in Lausanne for cryo-EM data collection. DCI is an initiative of the EPFL, University of Lausanne and University of Geneva. We also thank Kelvin Lau from PTPSP laboratory (EPFL) and the PCF laboratory (EPFL). This work was supported by the Swiss National Science Foundation (grant N° CRSII5_177195 and grant N° 310030_188548 to HS; grant N° 31003 A 173059 to CH), the Sino-Swiss Science and Technology Cooperation SSSTC (N° IZLCZ0_206089 to HS), the Fundação para a Ciência e Tecnologia for support through the MOSTMICRO-ITQB unit (UIDB/04612/2020 and UIDP/04612/2020 to AGD and IACP) and the Associated Laboratory LS4FUTURE (LA/P/0087/2020 to AGD and IACP). Publisher Copyright: © 2023, The Author(s).Organohalide-respiring bacteria are key organisms for the bioremediation of soils and aquifers contaminated with halogenated organic compounds. The major players in this process are respiratory reductive dehalogenases, corrinoid enzymes that use organohalides as substrates and contribute to energy conservation. Here, we present the structure of a menaquinol:organohalide oxidoreductase obtained by cryo-EM. The membrane-bound protein was isolated from Desulfitobacterium hafniense strain TCE1 as a PceA2B2 complex catalysing the dechlorination of tetrachloroethene. Two catalytic PceA subunits are anchored to the membrane by two small integral membrane PceB subunits. The structure reveals two menaquinone molecules bound at the interface of the two different subunits, which are the starting point of a chain of redox cofactors for electron transfer to the active site. In this work, the structure elucidates how energy is conserved during organohalide respiration in menaquinone-dependent organohalide-respiring bacteria.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNCimmino, LorenzoDuarte, Américo G.Ni, DongchunEkundayo, Babatunde E.Pereira, Inês A.C.Stahlberg, HenningHolliger, ChristofMaillard, Julien2024-04-02T23:47:43Z2023-122023-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/165734eng2041-1723PURE: 83672405https://doi.org/10.1038/s41467-023-42927-7info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:20:08Zoai:run.unl.pt:10362/165734Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:50:52.954240Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Structure of a membrane-bound menaquinol:organohalide oxidoreductase
title Structure of a membrane-bound menaquinol:organohalide oxidoreductase
spellingShingle Structure of a membrane-bound menaquinol:organohalide oxidoreductase
Cimmino, Lorenzo
Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
SDG 7 - Affordable and Clean Energy
title_short Structure of a membrane-bound menaquinol:organohalide oxidoreductase
title_full Structure of a membrane-bound menaquinol:organohalide oxidoreductase
title_fullStr Structure of a membrane-bound menaquinol:organohalide oxidoreductase
title_full_unstemmed Structure of a membrane-bound menaquinol:organohalide oxidoreductase
title_sort Structure of a membrane-bound menaquinol:organohalide oxidoreductase
author Cimmino, Lorenzo
author_facet Cimmino, Lorenzo
Duarte, Américo G.
Ni, Dongchun
Ekundayo, Babatunde E.
Pereira, Inês A.C.
Stahlberg, Henning
Holliger, Christof
Maillard, Julien
author_role author
author2 Duarte, Américo G.
Ni, Dongchun
Ekundayo, Babatunde E.
Pereira, Inês A.C.
Stahlberg, Henning
Holliger, Christof
Maillard, Julien
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Cimmino, Lorenzo
Duarte, Américo G.
Ni, Dongchun
Ekundayo, Babatunde E.
Pereira, Inês A.C.
Stahlberg, Henning
Holliger, Christof
Maillard, Julien
dc.subject.por.fl_str_mv Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
SDG 7 - Affordable and Clean Energy
topic Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
SDG 7 - Affordable and Clean Energy
description Funding Information: We thank the Dubochet Center for Imaging (DCI) in Lausanne for cryo-EM data collection. DCI is an initiative of the EPFL, University of Lausanne and University of Geneva. We also thank Kelvin Lau from PTPSP laboratory (EPFL) and the PCF laboratory (EPFL). This work was supported by the Swiss National Science Foundation (grant N° CRSII5_177195 and grant N° 310030_188548 to HS; grant N° 31003 A 173059 to CH), the Sino-Swiss Science and Technology Cooperation SSSTC (N° IZLCZ0_206089 to HS), the Fundação para a Ciência e Tecnologia for support through the MOSTMICRO-ITQB unit (UIDB/04612/2020 and UIDP/04612/2020 to AGD and IACP) and the Associated Laboratory LS4FUTURE (LA/P/0087/2020 to AGD and IACP). Funding Information: We thank the Dubochet Center for Imaging (DCI) in Lausanne for cryo-EM data collection. DCI is an initiative of the EPFL, University of Lausanne and University of Geneva. We also thank Kelvin Lau from PTPSP laboratory (EPFL) and the PCF laboratory (EPFL). This work was supported by the Swiss National Science Foundation (grant N° CRSII5_177195 and grant N° 310030_188548 to HS; grant N° 31003 A 173059 to CH), the Sino-Swiss Science and Technology Cooperation SSSTC (N° IZLCZ0_206089 to HS), the Fundação para a Ciência e Tecnologia for support through the MOSTMICRO-ITQB unit (UIDB/04612/2020 and UIDP/04612/2020 to AGD and IACP) and the Associated Laboratory LS4FUTURE (LA/P/0087/2020 to AGD and IACP). Publisher Copyright: © 2023, The Author(s).
publishDate 2023
dc.date.none.fl_str_mv 2023-12
2023-12-01T00:00:00Z
2024-04-02T23:47:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/165734
url http://hdl.handle.net/10362/165734
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2041-1723
PURE: 83672405
https://doi.org/10.1038/s41467-023-42927-7
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