Detalhes bibliográficos
| Ano de defesa: |
2011 |
| Autor(a) principal: |
PINHEIRO, Morganna Pollynne Nóbrega
 |
| Orientador(a): |
MELO FILHO, Péricles de Albuquerque |
| Banca de defesa: |
SANTOS, Roseane Cavalcanti,
CARVALHO, Reginaldo de,
MARTINS, Luiza Suely Semen |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal Rural de Pernambuco
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Melhoramento Genético de Plantas
|
| Departamento: |
Departamento de Agronomia
|
| País: |
Brasil
|
| Palavras-chave em Português: |
|
| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6486
|
Resumo: |
The cotton crop is present in several sectors, from agriculture to the industry, generating about 70% of employment to level of primary production, putting the cotton as a major international commodities. The Brazil has been increasing in terms of production, currently the fifth largest world producer and exporter quarter. Despite this, the management of the crop is very high and the main costs for machinery and to control pests and diseases. With the advent of the current practices of modern biotechnology, biological strategies for the control of pests and diseases have been used, including the use of plants genetically modified to contain genes that defend these evils. These genes, often regulated by constitutive expression promoters provide protection throughout the plant, although the levels are different in appearance tissue. In this case, the use of tissue-specific promoters to drive the sequence codante allows more direct action to protect and lowest power plant to promote target protein. The cotton crop is vulnerable to various pests that affect the reproductive structures, especially lepidopters and coleopters. With a view to ascertaining genes expressed in flower buds to subsequently use them in the area of trangenics, a subtractive cDNA library from flower buds built.We generated 768 sequences, having been formed 168 clusters with 126 contigs and 42 singlets. The in silico analysis were performed against the database of cotton (CottonDB) and Bank of Arapidopisis thaliana (TAIR) and many genes have been identified. From this analysis we selected six genes related to the development of eggs, fibers, pollen tube and pistil (ANTIFIB010, ASH, OVU, FIB010, FIBEARLY and GLUCANASE) to studies of semiquantitative RT-PCR. The results showed that the selected genes are expressed in all tissues studied (button, stem, leaf and root), however we could observe a higher level of expression in flower buds. |
