Funcionalidade das proteínas EF-Tu e 14-3-3 na virulência de Paracoccidioides brasiliensis

• Ano de defesa: 2015
• Autor(a) principal: Marcos, Caroline Maria [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Paracoccidioides brasiliensis; Paracoccidioidomicose; Micologia; Fungos; Virulencia (Microbiologia); Paracoccidioidomycosis
• Link de acesso: http://hdl.handle.net/11449/132196

Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis (PCM), a systemic mycosis with broad distribution in Latin America. The adhesion and host cell invasion are critical events involved in the infection and dissemination of the pathogen. Furthermore, they use their surface molecules to bind to extracellular matrix components to establish infection. The successful colonization of host tissues by the fungus is a complex event, usually involving a linker encoded by the pathogen (adhesins) and a cell receptor (often an extracellular matrix component). The identification of adhesion mechanisms, invasion or immune evasion by P. brasiliensis is extremely important and has been investigated in recent studies developed by several groups. In order to study the steps involved from the initial contact of P.brasiliensis until the culminating in its entry into the host cell through the functional characterization of P. brasiliensis membrane proteins, the main targets of interaction with host molecules, is of great importance. The elongation factor Tu (EF-Tu) belongs to the group of proteins called moonlighting such molecules have the ability to perform more than one function, and typically are located in different compartments of the cell. There are reports that EF-Tu of pathogens can act as a virulence factor. This protein has been previously identified in P. brasiliensis by proteomic analysis, but this has not yet been characterized as being interesting then the elucidation of its role in interacting of the fungus with epithelial cells. For this purpose the aim of this study was to produce the recombinant protein and its respective polyclonal antibody, verify your role in the fungus interaction with pneumocytes, extracellular matrix and plasminogen. Also in order to characterize the EF-Tu protein the present study aimed to obtain isolated with low expression of the gene encoding EF-Tu and with this verifies the role of EF-...