Caracterização e identificação de moléculas de superfície presentes em Paracoccidioides spp

• Ano de defesa: 2014
• Autor(a) principal: Oliveira, Haroldo Cesar de [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Fungos; Paracoccidioides brasiliensis; Paracoccidioidomicose; Micoses; Virulencia (Microbiologia); Fungi
• Link de acesso: http://hdl.handle.net/11449/123823; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/28-05-2015/000829993.pdf

The Paracoccidioides genus consists of dimorphic fungi, etiologic agents of paracoccidioidomycosis (PCM). Currently, phylogenetic studies divide the Paracoccidioides genus in two species named Paracoccidioides brasiliensis and Paracoccidioides lutzii. The first is divided in three phylogenetic species, S1, PS2 and PS3 and the second is named Pb01-like. The correct molecular taxonomy of this fungus has opened new possibilities for the study and understanding of their relationships with their hosts. The fungi of the Paracoccidioides genus have some features that allow their growth in adverse conditions provided by the host, which may contribute to disease development and it have mechanisms that enable them to adhere and invade host tissues. Adhesion is provided by a particular class of proteins present in the cell wall called adhesins, capable of mediating interactions with the fungal host tissues during infection. Differences in adhesion are responsible for increased virulence/pathogenicity of an isolate in relation to others. The goal of this study was to contribute to a better knowledge of the superficial components of the species of Paracoccidioides genus e its influence in the fungi virulence. For this, we analyzed the adhesion profile and the adhesins expression during the interaction of the fungi with the host, cloned and expressed the CS protein, in a heterologous system, which may be involved in virulence of Paracoccidioides spp. as well as characterize and identify molecules capable of inhibit the adhesion of Paracoccidioides spp. using a Phage Display system. So, when we evaluated the adhesion profile of the species P. brasiliensis and P. lutzii, as well as the analysis of the expression of genes coding adhesins by Real Time PCR, it has been founded a high heterogeneity of behavior in the different species, showing that the adhesins 14-3-3 and enolase are the most used ones adhesins by the pathogen, independent of the strain ...