Temperature and dehydration effects on respiratory and cutaneous water flux in the terrestrial toad, Rhinella schneideri (Anura, Bufonidae)

• Ano de defesa: 2018
• Autor(a) principal: Castro, Luis Miguel Senzano
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Anfíbio; Amphibians; Evaporative water loss; Skin resistance; Water uptake; Lung ventilation
• Link de acesso: http://hdl.handle.net/11449/154104

As ectotherms, amphibians may experience wide fluctuations in body temperature and, due to their high skin permeability, terrestrial species face a constant risk of desiccation. These two variables (temperature and dehydration) are centrally relevant for water balance regulation because they directly affect water flux through the integument, i.e. skin evaporative water loss (EWLSkin) and water uptake (WU). In addition, as nearly all anurans are lung breathers, the respiratory water loss (EWLResp) will add to EWLSkin. Although the contribution of EWLResp to total EWL (EWLTotal) is generally assumed to be negligible, the partitioning between EWLSkin and EWLResp varies among species and is affected by temperature and dehydration. Therefore, we investigated the combined effects of temperature and dehydration on the rates of EWL through the skin and the lungs of the terrestrial toad, Rhinella schneideri. Subsequently, we evaluated how water uptake (WU) through the pelvis skin was affected by temperature and dehydration. To this aim, we measured rates of EWLTotal and EWLSkin in intact and masked adult toads at 15, 25 and 35 oC under fully hydrated condition and dehydrated until they have lost 10% and 20% of their initial body mass. Masked toads were able to breath normally during the measurement of EWLSkin; EWLResp was calculated as EWLTotal minus EWLSkin. Rates of EWL were also determined using biophysical agar models of R. schneideri specimens, which allowed the estimation of skin resistance (Rs) to evaporation. WU rates were determined by measuring body mass gain against rehydration time of toads placed on a thin film of water. EWLSkin and EWLResp increased with temperature, however, this effect was much more pronounced for EWLResp than for EWLSkin and, as a result, the partitioning between cutaneous and respiratory water loss was significantly altered with temperature. Indeed, the contribution of EWLResp to EWLTotal increased from 2.44% at 15 oC to 8.1% at 35 oC. This result may be attributed to a limited capacity for EWLSkin regulation combined with a temperature induced increment in pulmonary ventilation resulting from the elevation in metabolic rate with temperature. The contribution of EWLSkin to EWLTotal decreased with dehydration which may be related to a loss in skin water content and the subsequent compression of cell layers, which limit water efflux to the environment. Therefore, the relative contribution of EWLResp augmented with temperature and dehydration accompanied by the corresponding decrease in the relative contribution of EWLSkin. Rates of WU increased with dehydration but not with temperature, which indicate the central role of the osmotic gradient in driving water flow through the anuran skin.