Imunofenótipos de linfócitos T no sangue e no liquor de cães com leishmaniose visceral: correlação com as lesões encefálicas

• Ano de defesa: 2013
• Autor(a) principal: Grano, Fernanda Grecco [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Imunologia veterinaria; Animais - Doenças; Citometria de fluxo; Leishmania; Sistema nervoso central; T-lymphocyte subsets; Celulas T
• Link de acesso: http://hdl.handle.net/11449/128070; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/16-09-2015/000819087.pdf

Visceral leishmaniasis (VL) is a disease causing several clinical manifestations in dogs, that can present from subclinical to generalized disorders, including neurological disorders. There are evidences of cerebral barriers involvement, such as the presence of inflammatory infiltrate with predominance of CD3+ T cells in the brain of infected dogs. Therefore, the aim of this study was to determine and to compare the immunophenotypes of T lymphocytes in the peripheral blood and in the cerebrospinal fluid (CSF) of dogs with VL and evaluate the brain lesions. It was detected that the double negative (DN) and double positive (DP) T cells were present in higher percentage in the CSF, with predominance of TCRαβ. Besides, the amount of blood T cells did not differ from those observed in the CSF, indicating that the blood-CSF barrier may be damaged, allowing the migration of cells from the blood to the CSF. Moreover, inflammatory infiltrate with predominance of lymphohistioplasmacytic cells was observed, mainly in leptomeninges. However, there was no correlation between the intensity of the inflammation in this area and the T cells. Furthermore, the positive correlation between intensity of the inflammation in the subependimal area and DN T cells in the CSF indicates that these cells also may reach the brain through the subependymal vessels. Together, the results contribute to explain the inflammation observed in the brain of dogs with VL, where the DN T cells may contribute to the neurological progression of the disease