Efeito do inseticida fipronil em abelhas africanizadas e na expressão de gene relacionado ao sistema imunológico

• Ano de defesa: 2014
• Autor(a) principal: Zaluski, Rodrigo [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Abelha-africana; Inseticidas; Expressão gênica; Toxicidade - Testes; Imunologia molecular; Apis mellifera adansonii
• Link de acesso: http://hdl.handle.net/11449/108833

In the present study we evaluated the in vitro toxicity (LD50) of fipronil and behavioral changes in adults Apis mellifera L. (Hymenoptera: Apidae) in contact and ingestion tests and the motor activity in bees exposed to the LD50 and sublethal dose (SD - 1/500 LD50). The SD (8μg L-1) was provided through the sugar syrup to hives and were evaluated the brood viability, population growth, behavior and expression of defensin 1 gene in adult bees. Fipronil was highly toxic by ingestion and contact, triggering agitation, seizures, tremors and paralysis. There was a reduction of motor activity of bees exposed to LD50 and SD. We observed reduced of hatching eggs, posture, larvae and pupae development and abandonment of colonies exposed to SD. Adult bees exposed to SD in swarms demonstrate inertia, without alterations in the defensin 1 gene expression. We concluded that fipronil have highly toxic effects to honeybees. bees