Expressão do RNAm e metilação do gene DKK2 em tecidos tumorais de câncer colorretal

• Ano de defesa: 2018
• Autor(a) principal: Mamelli, Ronaldo Eliezer [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: DKK2 gene; Colorectal cancer; Diagnosis; Prognosis; Gene DKK2; Câncer colorretal; Diagnóstico; Prognóstico
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=6441943; https://repositorio.unifesp.br/handle/11600/52534

BACKGROUND: Colorectal cancer (CRC) is the third most common neoplasm in the world. Methylation of tumor related genes in CpG islands can cause gene silencing and been involved in the development of cancer. The potential role of DKK2 as a biomarker for early diagnosis of CRC remains unclear. The aim of the study was to evaluate the profile of methylation and RNAm expression of DKK2 as potential predictors of CRC diagnosis and prognosis. PATIENTS AND METHODS: Expression of mRNAs encoding DKK2 in 35 CRC tissues was quantified using real-time polymerase chain reaction analysis. The DNA methylation was studied by High Resolution Melting (HRM) analysis. The general characteristics of the patients were collected. DKK2 methylation and expression were compared to clinical, pathological aspects and overall survival. RESULTS: Among the 35 patients studied, 18 were male, 10 were on ascending or transverse colon and 25 on descending or rectum. Among the 20 patients with high hipermethylation, 12 of them had mRNA low expression of DKK2. There was no significant association between DKK2 promoter methylation and DKK2 RNA expression and clinical or pathological features. DKK2 promoter methylation (p=0.154) and DKK2 RNA expression (p=0.345) did not show significant correlation with prognostic factors for overall survival in CRC patients. CONCLUSION: DKK2 promoter methylation and mRNA expression appears to be biomarkers of cancer diagnosis but not predictors of prognosis.