Avaliação das alterações das atividades de metalopeptidases no sistema nervoso central em modelos de privação de sono

• Ano de defesa: 2010
• Autor(a) principal: Visniauskas, Bruna [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Hormônio liberador de gonadotrofinas; Peptídeos opióides; Privação de sono paradoxal; Thimet oligopeptidase; GnRH; Enzima conversora de angiotensina-I; Peptidil dipeptidase A; Privação do sono; Memória; Angiotensin-I converting enzyme; Paradoxical sleep deprivation; Memory; Opioid peptides; Central nervous systems; Gonadotropin-releasing hormone; Sleep deprivation; Peptidyl-Dipeptidase A
• Link de acesso: http://repositorio.unifesp.br/handle/11600/9051

Neuropeptides have a fundamental role on sleep-wake cycle control and their actions are regulated by proteolytic processing. Proteolytic enzymes are essential for the metabolism of neuropeptides, either by release from active or inactive precursor protein, or the inactivation of active neuropeptides. Using fluorescence resonance energy transfer substrates, Western blot, specific inhibitors and real time PCR, we have demonstrated changes in the expression and activity of angiotensin I-converting enzyme (ACE - EC 3.4.15.1) and thimet oligopeptidase (EP24.15 – EC 3.4.24.15) in the central nervous system of rats submitted to paradoxical sleep deprivation. Male rats were distributed in 5 groups: control, paradoxical sleep deprivation during 96h (PSD96), and sleep recovery (recovery after sleep deprivation 24, 48 and 96h after PSD96). Alterations in the activities, expression and mRNA levels of ACE and EP24.15 were found in extracts of the hypothalamus, hippocampus, brainstem, cortex and striatum. Significant changes in these parameters (activity, mRNA levels, protein expression) were observed in groups PSD96 and recovery. Modifications on ACE and EP24.15 activities can result in alterations in the metabolism of angiotensins, bradykinin, opioid peptides (dynorphins and enkephalins), substance P and GnRH and be related to some of the physiological changes (stress, memory and cognition, nociception and endocrine changes) observed during and after sleep deprivation.