Avaliação da expressão gênica das vias de sinalização de insulina em células da granulosa provenientes de mulheres eutróficas e obesas com síndrome dos ovários policísticos em ciclos de fertilização in vitro

• Ano de defesa: 2015
• Autor(a) principal: Chehin, Mauricio Barbour [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: polycystic ovary syndrome; in vitro fertilization; obesity; insulin; granulosa cells; gene expression; síndrome do ovário policístico; fertilização in vitro; obesidade; insulina; células da granulosa; expressão gênica
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3213324; https://repositorio.unifesp.br/handle/11600/47583

Objective: To evaluate granulosa cell gene expression from insulin signaling pathway of Polycystic Ovarian Syndrome (PCOS) patients after In Vitro Fertilization (IVF) and compare the granulosa gene expression activity between eutrophic and obese women. Methods: Cross-sectional study with 15 PCOS patients, nine eutrophic and six obese, which were submitted to oocyte recovery to IVF and after the procedure had the granulosa cumulus cells removed from the oocyte to RNA extraction and later quantitative PCR array analysis of expression gene profile. The results were expressed by fold up or fold down of obese patients gene expression over eutrophic patients gene expression, fold ? 3 or ? 3 values and p ? 0,05 were considered statiscally significant. Results: The analyzed genes are overexpressed in obese compared to eutrophic women. There are 09 genes, BCL2L1, BRAF, CBL, DOK1, FBP1, FRS2, PCK2, RPS6KA1 and SORBS1 that presents fold ? 3 and p ? 0,05. Conclusions: In the group OB significantly overexpressed genes are responsible for the proliferation and differentiation of cumulus cells during oocyte maturation, insulin resistance, regulation of apoptosis, and glucose metabolism during early embryogenesis.