Padronização da estratégia de análise proteômica de células da granulosa de pacientes submetidas à fertilização in vitro

• Ano de defesa: 2014
• Autor(a) principal: Silva, Mayte Ugeda [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: granulosa cells; fertilization techniques; proteômica; fertilização in vitro; células da granulosa
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=1920464; https://repositorio.unifesp.br/handle/11600/46902

Objective: To establish the best conditions for proteomic analysis in granulosa cells from patients undergoing controlled ovarian stimulation for in vitro fertilization techniques. Methodology: techniques for quantification of samples occurred by the methods of Quibit, Bradford and BCA. The proteomic techniques were used for comparison - the bidimensional electrophoresis, the Shotgun and multiplex immunoassay. Results: The multiplex immunoassay method was the lower amount of protein (2.5 ?g) compared to bidimensional electrophoresis of 300 ?g and 50 ?g Shotgun was needed. Conclusion: The multiplex immunoassay was testing proteomics which required shorter execution time and a reduced amount of protein than another techiniques.