Caracterização química bioguiada pela atividade antioxidante de Erytrhoxylum mucronatum Benth. (Erythroxylaceae)

• Ano de defesa: 2018
• Autor(a) principal: Santana, Gracielle Silva
• Orientador(a): Duarte, Marcelo Cavalcante
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Programa de Pós-Graduação: Pós-Graduação em Ciências Farmacêuticas
• Palavras-chave em Português: Antioxidantes; Fenóis; Erythroxylum; Compostos fenólicos
• Palavras-chave em Inglês: Antioxidant; Phenolic compounds
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::FARMACOLOGIA
• Link de acesso: http://ri.ufs.br/jspui/handle/riufs/7603

The search for healing and the service of diseases through the ingestion of herbs and leaves may have been one of the first ways of using natural products. Scientists and the pharmaceutical industry have demonstrated intense in ventures researched for the purpose of discovery. Other species found in the flora of Sergipe are still unknown or little known scientifically, such as the Erythroxylum mucronatum species that has no reports in the literature of its use in folk medicine, as well as chemical and pharmacological studies have not yet been recorded. The objective of the present study was to carry out a chemical characterization bioguided by the antioxidant activity of this species. The antioxidant measurement for the crude ethanolic extract (EEB) and ethyl acetate (FAE), chloroform (FC) and hexane (FH) phases were tested, all of which presented antioxidant activity, with emphasis on FAE which showed to be the most active, through the methods of elimination of DPPH radicals (2,2-diphenyl-1-picryl-hydrazila (IC50 = 37.9 μg / mL), ABTS (2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid)) (IC50 = 452.8 μg / mL), NO (Nitric Oxide) (IC50 = 122.81 μg / mL) and reductive potential (FRAP), where it demonstrated activity superior to the Trolox® standard. The content of total phenolic compounds for AED was 348.85 ± 8.47 mg of gallic acid equivalent / g of the sample. A high-efficiency liquid chromatograph coupled to mass spectrometer (HPLC-MS) analysis identified 20 sets of phenolic present in FAE. Among the compounds identified, FAE20 was isolated and isolated by 1H and 13C nuclear magnetic resonance (RMN), termed quercetin-3-O-α-L-rhamnoside. Together, these results show that it is a studied and bioproductive species of phenolic compounds and that besides being an antioxidant, it has the potential to carry out new pharmacological studies.