Detalhes bibliográficos
| Ano de defesa: |
2013 |
| Autor(a) principal: |
Hirschmann, Lourdes Caruccio |
| Orientador(a): |
Brod, Claudiomar Soares |
| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Pelotas
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Parasitologia
|
| Departamento: |
Biologia
|
| País: |
BR
|
| Palavras-chave em Português: |
|
| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
|
| Link de acesso: |
https://guaiaca.ufpel.edu.br/handle/123456789/2329
|
Resumo: |
The canine visceral leishmaniasis (CVL) is a chronic zoonotic systemic disease, which affects numerous mammals, caused by protozoa Leishimania (Leishimania) chagasi which is transmitted by the hematophagy of the female phlebotominae Lutzomyia longipalis. The CVL shows vast geographical distribution and great lethality potential. Rio Grande do Sul was considered a non-endemic state. However, in 2008, autochthonous cases of the disease in Western cities of the state were reported. The aim of this study was to survey the presence of seropositive dogs in kennels in twelve municipalities of Rio Grande do Sul considering the clinical, epidemiological and laboratory aspects therefore being able to early detect a possible spreading of the disease encouraging prevention and controlling campaigns in order to avoid future outbreaks. This study was conducted in an area without diagnosis for the disease, collecting sampling material of 165 dogs from the cities of Alegrete, São Franscico de Assis, Santiago, Dom Pedrito, Bagé, Caçapava do Sul, Cachoeira do Sul, Piratini, Arroio grande, Capão do Leão, Rio Grande and Camaquã. Animals were submitted to general clinical evaluation and tegumentary and lymphadenopathy injuries. In the laboratory evaluation, CBC, parasitological and serological examinations were performed. The information of each animal was described in detail in the form of clinical examination and then submitted to EpiInfo version 6.04. Dermatological signs, normocytic normochromic anemia, platelet changes and neutrophilic leukocytosis were presented by the suspected and confirmed animals. Serological analysis was performed using the techniques of indirect immunofluorescence (IIF), enzyme linked immunosorbent assay (ELISA) and Dual Plate Platform (DPP). It was found the rate of 33.94% (56/165) reagents for Leishmania chagasi in the IIF, the DPP showed that 6.67% (11/165) were positive, while 3.03 % (5/165) were positive in both tests (IIF and DPP) and ELISA showed a 6.06% (11/165). In these confirmed by ELISA, five (5/10) were reagents in IIF and only three (3/10) were positive in DPP, resulting in three seropositive canines according to the updated protocol recommended by the Ministry of Health. A comparison of serological techniques demonstrated that DPP and IIF alone showed an accuracy of 65.45% while in the analysis of the same tests, considering ELISA as a gold standard, the accuracy of the DPP was 90.91% and IFF showed 66.60%. However, when considering only the positive results in DPP and IIF, the accuracy increased to 94.55%, with Kappa=0.375, that is, with considerable agreement. Concluding the investigation of non-endemic districts, five seropositive dogs were revealed: in Cachoeira do Sul (2), in São Francisco de Assis (1), in Dom Pedrito (1) and in Rio Grande (1). |
