PESQUISA DE COXIELLA BURNETTI E RICKETTSIA SPP. EM REBANHOS DE OVINOS PANTANEIROS DOS MUNICÍPIOS DE CAMPO GRANDE E TERENOS, MATO GROSSO DO SUL

• Ano de defesa: 2024
• Autor(a) principal: Mariana Ramos Santos
• Orientador(a): Alexsandra Rodrigues de Mendonça Favacho
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Fundação Universidade Federal de Mato Grosso do Sul
• País: Brasil
• Palavras-chave em Português: coxiella burnetti; rickettsia ssp; rebanhos ovinos; pantaneiros; campo grande; terenos
• Link de acesso: https://repositorio.ufms.br/handle/123456789/11000

Q fever and Brazilian spotted fever are zoonoses known worldwide, caused by bacteria of the genera Coxiella and Rickettsia, respectively, which present a global threat and have substantial impacts on human and animal health, as well as on the economy of animal breeding. With the objective of analyzing the participation of sheep in the epidemiology of diseases caused by C. burnetii and Rickettsia spp., a descriptive epidemiological study was developed on two properties: Sheep Farming Technological Center (CTO) of UNIDERP in Campo Grande and Fazenda Modelo of Embrapa Beef Cattle, in Terenos, both in the state of Mato Grosso do Sul, Brazil. Whole blood and blood-EDTA samples from 287 sheep were collected in September 2021, in both areas, followed by brief anamnesis and data collection from the herds. The serum samples were subjected to the Indirect Immunofluorescence Reaction (IFAT) to detect anti-C IgG antibodies. burnetii and anti-R. rickettsii. Of the 287 samples tested, 4 (1.4%) were reactive for C. burnetii, with titers equal to 64. For R. rickettsii, no sample was reactive. To investigate C. burnetii, nested PCR was performed using htpAB oligonucleotides, measuring 687bp and 440bp, in the first and second PCR, respectively. For the detection of Rickettsia sp. DNA, CS-78/CS-323 oligonucleotides were used, amplifying a 401bp fragment of the citrate synthase gene (gltA). The presence of PCR inhibitors was checked for IRBP amplification. Ectoparasites were not found at the time of the anamnesis. In PCR, the DNAs of C. burnetii and Rickettsia sp. were not amplified in any of the samples tested. The study showed the presence of anti-C. burnetii in Pantanal sheep in the tested herds, confirming for the first time that this agent is circulating among the herds and the absence of Rickettsia spp. in the two areas studied. An in-depth understanding of the presence of these pathogens in animal populations is crucial for promoting public health and single health, integrating aspects of animal, human and environmental health. Keywords: Q fever. Spotted fever. Surveillance. Zoonoses. One Health.