Estudo epidemiológico da coinfecção por toxoplasma gondii e pelo vírus da imunodeficiência felina em gatos domésticos (felis catus) em Goiânia, Goiás

• Ano de defesa: 2015
• Autor(a) principal: Costa, Rebeka Cristine de Bastos
• Orientador(a): Jayme, Valéria de Sá
• Banca de defesa: Jayme, Valéria de Sá, Silveira Neto, Osvaldo José da, Santin , Ana Paula Iglesias
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Ciência Animal (EVZ)
• Departamento: Escola de Veterinária e Zootecnia - EVZ (RG)
• País: Brasil
• Palavras-chave em Português: AIDS felina; ELISA indireto; Hemaglutinação indireta; Oocisto; PCR convencional; Toxoplasmose
• Palavras-chave em Inglês: Conventional PCR; Feline AIDS; Indirect ELISA; Indirect hemagglutination; Oocyst; Toxoplasmosis
• Área do conhecimento CNPq: MEDICINA VETERINARIA PREVENTIVA::DOENCAS INFECCIOSAS DE ANIMAIS
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/5115

Toxoplasmosis is a zoonotic disease in which mammals and birds can join the cycle as intermediate hosts, and felids as definitive hosts. Felis catus is recognized as the main responsible for the environmental contamination by Toxoplasma gondii oocysts. Serological diagnosis reveals little about the elimination of oocysts of T. gondii into the environment, principally by F. catus, which plays an important role in Public Health. There are few data on the frequency of feline toxoplasmosis in the State of Goiás. Therefore, the aim of this research was to evaluate the frequency of toxoplasmosis infection in domestic cats and their potential role in its transmission through the oocyst elimination into the environment and the respective factors associated with the infection. For this, we collected 102 blood samples and 98 fecal samples from 102 cats from Goiânia, State of Goiás. The animals were divided into groups according to age, gender and free access to the street or not. Indirect hemagglutination test was performed to determine the level of anti-T. gondii and indirect ELISA for the detection of infection by feline immunodeficiency virus (FIV). For search and detection of T. gondii oocysts elimination in the feces of cats we performed a centrifugal-flotation with Sheather's solution, subsequently we extracted DNA and used conventional PCR. The results showed that 18.63% (19/102) of the cats were positive for T. gondii, with titers ranging from 1:32 to 1: 8.192, while none of the fecal samples were positive in the PCR. The frequency of positive animals for FIV was 55.91% (52/93), and 18.28% (17/93) presented coinfection. By multivariate logistic regression we found the associated factors were the same for both infections, but one did not interfere with another. The factors associated with infection by T. gondii and feline immunodeficiency virus were free life and age under six months, since the sex was not statistically related to any of the illnesses.