Detalhes bibliográficos
| Ano de defesa: |
2015 |
| Autor(a) principal: |
Neves, Rogério Coutinho das
 |
| Orientador(a): |
Junqueira-Kipnis, Ana Paula
|
| Banca de defesa: |
Cardoso, Juliana Lamaro,
Vieira, José Daniel Gonçalves,
Junqueira-Kipnis, Ana Paula |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Goiás
|
| Programa de Pós-Graduação: |
Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)
|
| Departamento: |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://repositorio.bc.ufg.br/tede/handle/tede/5584
|
Resumo: |
Outbreaks caused by atypical mycobacteria are considered as an emerging problem in Brazil and Goias, being frequently associated with invasive procedures, such as laparoscopy, arthroscopy, and aesthetic surgeries among others. Treatment of mycobacteriosis requires highly toxic drugs with doubtful sterilizing effects. Consequently, several studies aim to search for new drugs or molecules with anti-mycobacterial biological activities. The objective of this study was to prospect biomolecules from the venom of the social wasp Polybia paulista (Vespoidea, Vespidae, Polistinae) and from the Tityus sp scorpion with antimycobacteria activity against Mycobacterium abscessus subsp. bolletii (GO06). Peptides from the wasp and scorpion venoms were evaluated in broth microdilution assay and in macrophage infected cultures to determine minimum inhibitory concentration. Peptides with antimycobacterial activity were further evaluated by scanning electron microscopy of M. abscessus subsp. bolletii treated cultures. The peptide toxicities were evaluated on macrophage cultures. Peptides Agelaia-MP, Polybia-MPII, and AVA, derived from wasp and peptides AMP 1, 2, 4, 6, and 8 derived from scorpion presented antimycobacterial activities. AVA and AMP 4 peptides were not toxic for macrophages. In this work three peptides derived from the wasp venom and five peptides derived from the scorpion venom were identified as having antimycobacterial activity. |
