Emprego das técnicas de pcr e vidas na determinação da escherichia coli o157:H7 em queijos minas frescal em feiras livres e estabelecimentos sob inspeção federal

• Ano de defesa: 2009
• Autor(a) principal: CARVALHO, Rosangela Nunes
• Orientador(a): OLIVEIRA, Antonio Nonato de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Mestrado em Ciência Animal
• Departamento: Ciências Agrárias - Veterinaria
• País: BR
• Palavras-chave em Português: queijo Minas Frescal; STEC; PCR; VIDAS ECO O157®.
• Palavras-chave em Inglês: Minas Frescal cheese; STEC; PCR; VIDAS ECO O157®
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tde/932

Escherichia coli O157:H7 is a serotype that belongs to Shiga toxin-producing Escherichia coli group and it has been incriminated in outbreaks of food poisoning and also as cause of sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. In Brazil, there is no data available about outbreaks caused by E. coli O157:H7, but this pathogen has been found often in cattle feces. Taking into consideration the high susceptibility of Minas Frescal cheese to the contamination by E. coli O157:H7 and also considering that milk is frequently associated with outbreaks, the aim of this research was to determinate the occurrence of the pathogen in Minas Frescal cheese, and also to compare techniques for this bacterium detection, such as PCR (Polymerase Chain Reaction) and VIDAS ECO O157® (bioMérieux, Lyon, France) test. Thirty cheese samples were collected from open-air markets in Goiânia and thirty samples were collected from establishments under Federal Inspection located in Goiás that trade their products in supermarkets in Goiânia. The MPN (More probable number) of Thermo-tolerant Coliforms and MPN of E. coli were also determined from the samples. From those samples collected in open air markets, E. coli O157:H7 was detected in 6,67% and 23,33% when the technique used was VIDAS ECO O157® and PCR, respectively. From those samples collected in establishments under Federal Inspection, the bacterium was not detected. There was no significant difference (p>0,05) between VIDAS ECO O157® and PCR for pathogen detection in samples from open air market and establishments under Federal Inspection. No significant difference (p> 0,05) was observed in samples collected from open air market and industries under Federal Inspection when VIDAS ECO O157® was used to E.coli O157:H7 detection. When PCR technique was applied, there was significant difference (p< 0,05) between samples origin. In 86,67% of samples collected from open air markets, Thermo-tolerant Coliforms were detected over the level established in Brazilian legislation, but in samples collected from industries under Federal Inspection, this value was 10%. The Minas Frescal cheese samples collected from open air market were more contaminated by E.coli than those collected from establishments under Federal Inspection and commercialized in supermarkets.