Produção de proteínas recombinantes do domínio III da proteína e do vírus dengue em Escherichia coli

• Ano de defesa: 2011
• Autor(a) principal: Duarte, Aíla Maria Souza Fontenele
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual do Ceará
• Palavras-chave em Português: Biotecnologia; Dengue; Proteína recombinante; Proteínas
• Link de acesso: https://siduece.uece.br/siduece/trabalhoAcademicoPublico.jsf?id=71024

<span class="markedContent" id="page52R_mcid2"><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*133.60px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03009);" role="presentation" dir="ltr">A dengue é hoje a arbovirose de maior relevância no contexto mundial. Segundo a </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*147.40px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03745);" role="presentation" dir="ltr">Organização Mundial da Saúde mais de 3,6 bilhões de pessoas estão vivendo em </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*161.20px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00151);" role="presentation" dir="ltr">rea de risco, principalmente nas regiões tropical e subtropical do planeta. Com essa</span> <span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00037);" role="presentation" dir="ltr">distribuição</span><span style="left: calc(var(--scale-factor)*145.84px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*155.72px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.996911);" role="presentation" dir="ltr">geográfica,</span><span style="left: calc(var(--scale-factor)*214.93px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*224.78px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00185);" role="presentation" dir="ltr">50</span><span style="left: calc(var(--scale-factor)*238.16px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*248.03px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">a</span><span style="left: calc(var(--scale-factor)*254.70px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*264.56px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997355);" role="presentation" dir="ltr">100</span><span style="left: calc(var(--scale-factor)*284.54px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*294.29px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00022);" role="presentation" dir="ltr">milhões</span><span style="left: calc(var(--scale-factor)*335.65px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*345.48px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00185);" role="presentation" dir="ltr">de</span><span style="left: calc(var(--scale-factor)*358.86px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*368.72px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999143);" role="presentation" dir="ltr">novos</span><span style="left: calc(var(--scale-factor)*400.73px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*410.56px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00201);" role="presentation" dir="ltr">casos</span><span style="left: calc(var(--scale-factor)*441.97px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*451.68px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00131);" role="presentation" dir="ltr">são</span><span style="left: calc(var(--scale-factor)*471.06px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*480.92px); top: calc(var(--scale-factor)*175.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00061);" role="presentation" dir="ltr">notificados </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999499);" role="presentation" dir="ltr">anualmente.</span><span style="left: calc(var(--scale-factor)*151.14px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*160.15px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">A</span><span style="left: calc(var(--scale-factor)*168.16px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*177.05px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00227);" role="presentation" dir="ltr">falta</span><span style="left: calc(var(--scale-factor)*199.79px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*208.70px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00185);" role="presentation" dir="ltr">de</span><span style="left: calc(var(--scale-factor)*222.08px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*231.11px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.995641);" role="presentation" dir="ltr">controle</span><span style="left: calc(var(--scale-factor)*273.62px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*282.65px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997594);" role="presentation" dir="ltr">dos</span><span style="left: calc(var(--scale-factor)*301.96px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*310.94px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999124);" role="presentation" dir="ltr">vetores</span><span style="left: calc(var(--scale-factor)*350.27px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*359.47px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00213);" role="presentation" dir="ltr">Aedes</span><span style="left: calc(var(--scale-factor)*393.58px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*402.57px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.994804);" role="presentation" dir="ltr">aegypti</span><span style="left: calc(var(--scale-factor)*441.08px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*450.04px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">e</span><span style="left: calc(var(--scale-factor)*456.71px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*465.74px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998305);" role="presentation" dir="ltr">albopictus,</span><span style="left: calc(var(--scale-factor)*523.02px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*532.20px); top: calc(var(--scale-factor)*188.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">a</span> <span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*202.60px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03223);" role="presentation" dir="ltr">inexistência de terapias antivirais eficazes e de vacinas hábeis provoca o aumento </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*216.40px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.07621);" role="presentation" dir="ltr">do número de casos da doença, suscitando o aparecimento da dengue severa.</span> <span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*230.20px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.06969);" role="presentation" dir="ltr">Aproximadamente 60% do número de casos de dengue registrados na América </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00065);" role="presentation" dir="ltr">latina</span><span style="left: calc(var(--scale-factor)*113.82px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*124.07px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997943);" role="presentation" dir="ltr">ocorrem</span><span style="left: calc(var(--scale-factor)*167.99px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*178.25px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.993761);" role="presentation" dir="ltr">no</span><span style="left: calc(var(--scale-factor)*191.52px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*201.62px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.0006);" role="presentation" dir="ltr">Brasil.</span><span style="left: calc(var(--scale-factor)*234.98px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*245.33px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">O</span><span style="left: calc(var(--scale-factor)*254.67px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*264.88px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998589);" role="presentation" dir="ltr">objetivo</span><span style="left: calc(var(--scale-factor)*306.19px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*316.42px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998393);" role="presentation" dir="ltr">desse</span><span style="left: calc(var(--scale-factor)*348.40px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*358.62px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999157);" role="presentation" dir="ltr">trabalho</span><span style="left: calc(var(--scale-factor)*401.97px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*412.09px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00435);" role="presentation" dir="ltr">foi</span><span style="left: calc(var(--scale-factor)*424.83px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*434.99px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.996559);" role="presentation" dir="ltr">produzir</span><span style="left: calc(var(--scale-factor)*478.20px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*488.33px); top: calc(var(--scale-factor)*244.00px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00127);" role="presentation" dir="ltr">proteínas</span> <span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*257.71px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03032);" role="presentation" dir="ltr">recombinantes em</span><span style="left: calc(var(--scale-factor)*186.83px); top: calc(var(--scale-factor)*257.71px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*193.25px); top: calc(var(--scale-factor)*257.71px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03595);" role="presentation" dir="ltr">Escherichia coli</span><span style="left: calc(var(--scale-factor)*279.63px); top: calc(var(--scale-factor)*257.71px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*286.03px); top: calc(var(--scale-factor)*257.71px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.07515);" role="presentation" dir="ltr">para servirem como insumo na produção de </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*271.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00753);" role="presentation" dir="ltr">vacinas, kits de diagnóstico e, ferramenta em estudos imunoepidemiológicos. Para a</span><br role="presentation"/><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00011);" role="presentation" dir="ltr">produção</span><span style="left: calc(var(--scale-factor)*135.17px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*143.11px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997594);" role="presentation" dir="ltr">das</span><span style="left: calc(var(--scale-factor)*162.42px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*170.33px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.99887);" role="presentation" dir="ltr">proteínas</span><span style="left: calc(var(--scale-factor)*220.32px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*228.23px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999359);" role="presentation" dir="ltr">recombinantes,</span><span style="left: calc(var(--scale-factor)*310.24px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*318.05px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00232);" role="presentation" dir="ltr">fragmentos</span><span style="left: calc(var(--scale-factor)*378.23px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*386.14px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999459);" role="presentation" dir="ltr">gênicos</span><span style="left: calc(var(--scale-factor)*427.49px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*435.42px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.993761);" role="presentation" dir="ltr">do</span><span style="left: calc(var(--scale-factor)*448.69px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*456.64px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999013);" role="presentation" dir="ltr">domínio</span><span style="left: calc(var(--scale-factor)*499.30px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*507.23px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.0042);" role="presentation" dir="ltr">III</span><span style="left: calc(var(--scale-factor)*517.27px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*525.08px); top: calc(var(--scale-factor)*285.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.993761);" role="presentation" dir="ltr">da </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*299.23px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03186);" role="presentation" dir="ltr">proteína E dos vírus dengue 2 e 3, foram amplificados a partir do cDNA por PCR e </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*312.91px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03539);" role="presentation" dir="ltr">clonados nos vetores pGEM-Teasy, pAE e pET28a em</span><span style="left: calc(var(--scale-factor)*385.35px); top: calc(var(--scale-factor)*312.91px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*390.10px); top: calc(var(--scale-factor)*312.91px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.01065);" role="presentation" dir="ltr">Escherichia coli.</span><span style="left: calc(var(--scale-factor)*477.74px); top: calc(var(--scale-factor)*312.91px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*482.28px); top: calc(var(--scale-factor)*312.91px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.02064);" role="presentation" dir="ltr">A proteína</span> <span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*326.83px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03452);" role="presentation" dir="ltr">expressa fusionada a cauda de 6-histidinas foi purificada através de cromatografia </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00185);" role="presentation" dir="ltr">de</span><span style="left: calc(var(--scale-factor)*98.48px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*110.51px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998308);" role="presentation" dir="ltr">afinidade</span><span style="left: calc(var(--scale-factor)*159.16px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*171.18px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00185);" role="presentation" dir="ltr">ao</span><span style="left: calc(var(--scale-factor)*184.56px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*196.58px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999847);" role="presentation" dir="ltr">Ni</span><span style="left: calc(var(--scale-factor)*207.89px); top: calc(var(--scale-factor)*338.11px); font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.981863);" role="presentation" dir="ltr">2+</span><span style="left: calc(var(--scale-factor)*217.01px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">.</span><span style="left: calc(var(--scale-factor)*220.35px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*232.37px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00023);" role="presentation" dir="ltr">Os</span><span style="left: calc(var(--scale-factor)*247.71px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*259.72px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00045);" role="presentation" dir="ltr">resultados</span><span style="left: calc(var(--scale-factor)*315.12px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*327.11px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00038);" role="presentation" dir="ltr">obtidos</span><span style="left: calc(var(--scale-factor)*365.83px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*377.82px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00185);" role="presentation" dir="ltr">no</span><span style="left: calc(var(--scale-factor)*391.20px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*403.23px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.9984);" role="presentation" dir="ltr">seqüenciamento</span><span style="left: calc(var(--scale-factor)*491.17px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*503.22px); top: calc(var(--scale-factor)*340.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999346);" role="presentation" dir="ltr">gênico</span> <span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*354.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.02084);" role="presentation" dir="ltr">demonstraram que a sequência de 848pb tinha 99% de homologia com sequências </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*368.23px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00719);" role="presentation" dir="ltr">gênicas codificadoras do domínio III da proteína E dos vírus dengue, depositadas no</span> <span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*382.03px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.02184);" role="presentation" dir="ltr">banco de dados do NCBI. O alinhamento da sequência de aminoácidos revelou um </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*395.83px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03191);" role="presentation" dir="ltr">grau de similaridade significativo com as seqüências previamente  escritas, com a</span> <span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*409.63px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.02082);" role="presentation" dir="ltr">ocorrência de apenas uma substituição na posição 140, confirmando que o produto</span> <span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*423.43px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.05076);" role="presentation" dir="ltr">de PCR amplificado corresponde ao domínio III da proteína E do vírus dengue. A </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00061);" role="presentation" dir="ltr">proteína</span><span style="left: calc(var(--scale-factor)*129.18px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*136.70px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998657);" role="presentation" dir="ltr">expressa</span><span style="left: calc(var(--scale-factor)*185.34px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*192.81px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999436);" role="presentation" dir="ltr">apresentou</span><span style="left: calc(var(--scale-factor)*252.85px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*260.31px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997533);" role="presentation" dir="ltr">peso</span><span style="left: calc(var(--scale-factor)*286.28px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*293.74px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998045);" role="presentation" dir="ltr">molecular</span><span style="left: calc(var(--scale-factor)*345.67px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*353.08px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00185);" role="presentation" dir="ltr">de</span><span style="left: calc(var(--scale-factor)*366.46px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*373.93px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.0038);" role="presentation" dir="ltr">aproximadamente</span><span style="left: calc(var(--scale-factor)*470.37px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*477.86px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.993761);" role="presentation" dir="ltr">31</span><span style="left: calc(var(--scale-factor)*491.13px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*498.59px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999863);" role="presentation" dir="ltr">kDa.</span><span style="left: calc(var(--scale-factor)*523.26px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*530.88px); top: calc(var(--scale-factor)*437.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">A</span> <span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00018);" role="presentation" dir="ltr">integridade</span><span style="left: calc(var(--scale-factor)*144.52px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*153.90px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999737);" role="presentation" dir="ltr">estrutural</span><span style="left: calc(var(--scale-factor)*203.92px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*213.37px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00185);" role="presentation" dir="ltr">da</span><span style="left: calc(var(--scale-factor)*226.75px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*236.14px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00061);" role="presentation" dir="ltr">proteína</span><span style="left: calc(var(--scale-factor)*280.21px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*289.72px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998413);" role="presentation" dir="ltr">recombinante</span><span style="left: calc(var(--scale-factor)*362.33px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*372.22px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998029);" role="presentation" dir="ltr">denominada,</span><span style="left: calc(var(--scale-factor)*441.50px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*450.94px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00063);" role="presentation" dir="ltr">6His-E2DIII</span><span style="left: calc(var(--scale-factor)*512.40px); top: calc(var(--scale-factor)*455.61px); font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr">2</span><span style="left: calc(var(--scale-factor)*516.87px); top: calc(var(--scale-factor)*455.61px); font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*526.20px); top: calc(var(--scale-factor)*451.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00435);" role="presentation" dir="ltr">foi </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00025);" role="presentation" dir="ltr">demonstrada</span><span style="left: calc(var(--scale-factor)*155.18px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*162.89px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997443);" role="presentation" dir="ltr">por</span><span style="left: calc(var(--scale-factor)*180.19px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*187.82px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00435);" role="presentation" dir="ltr">meio</span><span style="left: calc(var(--scale-factor)*213.95px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*221.65px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00185);" role="presentation" dir="ltr">de</span><span style="left: calc(var(--scale-factor)*235.03px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*242.50px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.0112);" role="presentation" dir="ltr">Western</span><span style="left: calc(var(--scale-factor)*287.46px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*295.02px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997833);" role="presentation" dir="ltr">blotting,</span><span style="left: calc(var(--scale-factor)*336.97px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*344.67px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00263);" role="presentation" dir="ltr">sendo</span><span style="left: calc(var(--scale-factor)*377.46px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*385.17px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.99871);" role="presentation" dir="ltr">reconhecida</span><span style="left: calc(var(--scale-factor)*450.48px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*458.19px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00367);" role="presentation" dir="ltr">por</span><span style="left: calc(var(--scale-factor)*475.60px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*483.60px); top: calc(var(--scale-factor)*464.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998284);" role="presentation" dir="ltr">anticorpos</span> <span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*478.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.06874);" role="presentation" dir="ltr">policlonais anti-proteína E do DENV, anticorpo anti-proteína recombinante 6His- </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*492.45px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00211);" role="presentation" dir="ltr">E2DIII</span><span style="left: calc(var(--scale-factor)*118.58px); top: calc(var(--scale-factor)*497.01px); font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.95658);" role="presentation" dir="ltr">2,</span><span style="left: calc(var(--scale-factor)*125.26px); top: calc(var(--scale-factor)*497.01px); font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*129.98px); top: calc(var(--scale-factor)*492.45px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.02792);" role="presentation" dir="ltr">anticorpos policlonais anti-flavivirus do grupo B, anticorpo monoclonal anti-</span><br role="presentation"/><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*506.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997174);" role="presentation" dir="ltr">His</span><span style="left: calc(var(--scale-factor)*102.38px); top: calc(var(--scale-factor)*510.81px); font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr">6</span><span style="left: calc(var(--scale-factor)*106.85px); top: calc(var(--scale-factor)*510.81px); font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*113.78px); top: calc(var(--scale-factor)*506.25px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.1026);" role="presentation" dir="ltr">comercial da GE e anticorpo humano de soro de paciente infectado com</span> <span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*520.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.0626);" role="presentation" dir="ltr">dengue. Portanto, a proteína E expressa é imunogênica sendo possível seu uso </span><span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*533.85px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999719);" role="presentation" dir="ltr">como insumo para kits de diagnóstico e de vacinas.</span></span><span class="markedContent" id="page52R_mcid3"></span><span class="markedContent" id="page52R_mcid4"> <span style="left: calc(var(--scale-factor)*85.10px); top: calc(var(--scale-factor)*567.45px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.05518);" role="presentation" dir="ltr">Palavras-chave</span><span style="left: calc(var(--scale-factor)*172.49px); top: calc(var(--scale-factor)*567.45px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999489);" role="presentation" dir="ltr">: Dengue, Proteína recombinante, expressão em procarioto.</span></span>