Efeito do alendronato no desenvolvimento da face: um estudo histométrico e imunohistoquímico

• Ano de defesa: 2019
• Autor(a) principal: Gomes, Shaiene Patricia
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Odontologia Clínica; UP
• Palavras-chave em Português: Odontologia; Alendronato - Odontologia; Palato; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2160

Little is known about the effects of sodium Alendronate on the development of the palatal raphe and nasal cavity in the neonatal period. Some studies have indicated that this drug may interfere with cartilaginous growth and promote the development of cartilage. This study evaluated the development and expansion of the palatal raphe in newborn rats 2 and 7 days old using HIH immunoexpression. In addition, on the 7th day of life, the presence of IHH, FGF-23 and Na+K+ATPase was determined in the nasal concha and cartilage, and the presence of Na+K+ATPase immunopresence, in the respiratory tract epithelium. Thirty-two neonatal rats were used, which were randomly assigned to two groups, the control group (C) and the test (ALN). The groups were divided into two subgroups for euthanasia at 2 and 7 days of age. After euthanasia, immunohistochemistry was used to analyze the expression of IHH, FGF-23 and Na+K+ATPase proteins, and histology was used to describe the topography. On the second day, the histological aspects of the palate in the control group revealed the presence of fibrous connective tissue surrounded by hyaline cartilaginous tissue. At the same time, HHI was expressed in a few cells in the mesenchyme of the cartilage, as well as in some of the chondrocytes. On the 7th day, many chondrocytic cells were separated by dense connective tissue, but IHH was scarce. Specimens of animals that received ALN had similar patterns when compared to the control group. However, several cells that make up the mesenchyme were associated with the intense IHH immunoexpression. On the seventh day, in the test group, chondroid staining revealed that a large area of hypertrophic chondrocytes formed well-characterized isogenic areas. Circumscribing most chondrocytes, there were four to six layers of hyaline cartilage and a mesenchyme all along the length of the midpalatal area. At that time, there was no bone deposition in these specimens. It is concluded that alendronate promoted chondroid expansion of palatine raphe in neonatal rats, associated with the increase of HIH immunoexpression in the chondroid areas of the palatine raphe. In nasal concha, it increased the immunoexpression of HHI and increased the expansivity, associated with suppression of FGF-23, which is a functional opponent of IHH. Also, specimens receiving alendronate had the Na + K + ATPase pump inhibited in the respiratory epithelium, both in the nasal cavity lining and in the maxillary sinus.