Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis

Bibliographic Details
Main Author: Rudkin, Fiona M.
Publication Date: 2018
Other Authors: Raziunaite, Ingrida, Workman, Hillary, Essono, Sosthene, Belmonte, Rodrigo, MacCallum, Donna M., Johnson, Elizabeth M., Silva, Lisete M., Palma, Angelina S., Feizi, Ten, Jensen, Allan, Erwig, Lars P., Gow, Neil A.R.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/127783
Summary: FCT Investigator IF/00033/2012. Wellcome Trust (086827, 075470, 099215, 099197 and 101873) Wellcome Trust ISSF award (105625), MRC CiC (MC_PC_14114) and MRC Centre for Medical Mycology and University of Aberdeen for funding and a Wellcome Trust Strategic Award (097377). Wellcome Trust grant 099197MA .
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network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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spelling Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasisChemistry(all)Biochemistry, Genetics and Molecular Biology(all)Physics and Astronomy(all)SDG 3 - Good Health and Well-beingFCT Investigator IF/00033/2012. Wellcome Trust (086827, 075470, 099215, 099197 and 101873) Wellcome Trust ISSF award (105625), MRC CiC (MC_PC_14114) and MRC Centre for Medical Mycology and University of Aberdeen for funding and a Wellcome Trust Strategic Award (097377). Wellcome Trust grant 099197MA .The high global burden of over one million annual lethal fungal infections reflects a lack of protective vaccines, late diagnosis and inadequate chemotherapy. Here, we have generated a unique set of fully human anti-Candida monoclonal antibodies (mAbs) with diagnostic and therapeutic potential by expressing recombinant antibodies from genes cloned from the B cells of patients suffering from candidiasis. Single class switched memory B cells isolated from donors serum-positive for anti-Candida IgG were differentiated in vitro and screened against recombinant Candida albicans Hyr1 cell wall protein and whole fungal cell wall preparations. Antibody genes from Candida-reactive B cell cultures were cloned and expressed in Expi293F human embryonic kidney cells to generate a panel of human recombinant anti-Candida mAbs that demonstrate morphology-specific, high avidity binding to the cell wall. The species-specific and pan-Candida mAbs generated through this technology display favourable properties for diagnostics, strong opsono-phagocytic activity of macrophages in vitro, and protection in a murine model of disseminated candidiasis.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNRudkin, Fiona M.Raziunaite, IngridaWorkman, HillaryEssono, SostheneBelmonte, RodrigoMacCallum, Donna M.Johnson, Elizabeth M.Silva, Lisete M.Palma, Angelina S.Feizi, TenJensen, AllanErwig, Lars P.Gow, Neil A.R.2021-11-15T23:33:05Z2018-12-012018-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/127783eng2041-1723PURE: 16665892https://doi.org/10.1038/s41467-018-07738-1info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:57:12Zoai:run.unl.pt:10362/127783Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:28:11.087504Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis
title Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis
spellingShingle Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis
Rudkin, Fiona M.
Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
SDG 3 - Good Health and Well-being
title_short Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis
title_full Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis
title_fullStr Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis
title_full_unstemmed Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis
title_sort Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis
author Rudkin, Fiona M.
author_facet Rudkin, Fiona M.
Raziunaite, Ingrida
Workman, Hillary
Essono, Sosthene
Belmonte, Rodrigo
MacCallum, Donna M.
Johnson, Elizabeth M.
Silva, Lisete M.
Palma, Angelina S.
Feizi, Ten
Jensen, Allan
Erwig, Lars P.
Gow, Neil A.R.
author_role author
author2 Raziunaite, Ingrida
Workman, Hillary
Essono, Sosthene
Belmonte, Rodrigo
MacCallum, Donna M.
Johnson, Elizabeth M.
Silva, Lisete M.
Palma, Angelina S.
Feizi, Ten
Jensen, Allan
Erwig, Lars P.
Gow, Neil A.R.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Rudkin, Fiona M.
Raziunaite, Ingrida
Workman, Hillary
Essono, Sosthene
Belmonte, Rodrigo
MacCallum, Donna M.
Johnson, Elizabeth M.
Silva, Lisete M.
Palma, Angelina S.
Feizi, Ten
Jensen, Allan
Erwig, Lars P.
Gow, Neil A.R.
dc.subject.por.fl_str_mv Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
SDG 3 - Good Health and Well-being
topic Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
SDG 3 - Good Health and Well-being
description FCT Investigator IF/00033/2012. Wellcome Trust (086827, 075470, 099215, 099197 and 101873) Wellcome Trust ISSF award (105625), MRC CiC (MC_PC_14114) and MRC Centre for Medical Mycology and University of Aberdeen for funding and a Wellcome Trust Strategic Award (097377). Wellcome Trust grant 099197MA .
publishDate 2018
dc.date.none.fl_str_mv 2018-12-01
2018-12-01T00:00:00Z
2021-11-15T23:33:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/127783
url http://hdl.handle.net/10362/127783
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2041-1723
PURE: 16665892
https://doi.org/10.1038/s41467-018-07738-1
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833596716656361472

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