Catalytic peptide-based coacervates for enhanced function through structural organization and substrate specificity

Bibliographic Details
Main Author: Reis, David Q.P.
Publication Date: 2024
Other Authors: Pereira, Sara, Ramos, Ana P., Pereira, Pedro M., Morgado, Leonor, Calvário, Joana, Henriques, Adriano O., Serrano, Mónica, Pina, Ana S.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/178560
Summary: This work was partially supported by PPBI - Portuguese Platform of BioImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE - \u201COr\u00E7amento de Estado\u201D and by european funds from FEDER - \u201CFundo Europeu de Desenvolvimento Regional. La Caixa Junior Leader Fellowship (LCF/BQ/PI20/11760012) financed by\u201Dla Caixa\u201D Foundation (ID 100010434) and a Maratona da Sa\u00FAde award. The NMR spectrometers at CERMAX, ITQB-NOVA, Oeiras are funded by FCT through project AAC 01/SAICT/2016, while those from FCT-NOVA are part of the National NMR Network and are supported by FCT (ROTEIRO/0031/2013) cofounded by FEDER through COMPETE 2020, POCI, PORL and FCT through PIDDAC. Publisher Copyright: © The Author(s) 2024.
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spelling Catalytic peptide-based coacervates for enhanced function through structural organization and substrate specificityChemistry(all)Biochemistry, Genetics and Molecular Biology(all)Physics and Astronomy(all)This work was partially supported by PPBI - Portuguese Platform of BioImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE - \u201COr\u00E7amento de Estado\u201D and by european funds from FEDER - \u201CFundo Europeu de Desenvolvimento Regional. La Caixa Junior Leader Fellowship (LCF/BQ/PI20/11760012) financed by\u201Dla Caixa\u201D Foundation (ID 100010434) and a Maratona da Sa\u00FAde award. The NMR spectrometers at CERMAX, ITQB-NOVA, Oeiras are funded by FCT through project AAC 01/SAICT/2016, while those from FCT-NOVA are part of the National NMR Network and are supported by FCT (ROTEIRO/0031/2013) cofounded by FEDER through COMPETE 2020, POCI, PORL and FCT through PIDDAC. Publisher Copyright: © The Author(s) 2024.Liquid-liquid phase separation (LLPS) in living cells provides innovative pathways for synthetic compartmentalized catalytic systems. While LLPS has been explored for enhancing enzyme catalysis, its potential application to catalytic peptides remains unexplored. Here, we demonstrate the use of coacervation, a key LLPS feature, to constrain the conformational flexibility of catalytic peptides, resulting in structured domains that enhance peptide catalysis. Using the flexible catalytic peptide P7 as a model, we induce reversible biomolecular coacervates with structured peptide domains proficient in hydrolyzing phosphate ester molecules and selectively sequestering phosphorylated proteins. Remarkably, these coacervate-based microreactors exhibit a 15,000-fold increase in catalytic efficiency compared to soluble peptides. Our findings highlight the potential of a single peptide to induce coacervate formation, selectively recruit substrates, and mediate catalysis, enabling a simple design for low-complexity, single peptide-based compartments with broad implications. Moreover, LLPS emerges as a fundamental mechanism in the evolution of chemical functions, effectively managing conformational heterogeneity in short peptides and providing valuable insights into the evolution of enzyme activity and catalysis in prebiotic chemistry.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)UCIBIO - Applied Molecular Biosciences UnitFaculdade de Ciências e Tecnologia (FCT)RUNReis, David Q.P.Pereira, SaraRamos, Ana P.Pereira, Pedro M.Morgado, LeonorCalvário, JoanaHenriques, Adriano O.Serrano, MónicaPina, Ana S.2025-02-06T21:19:55Z2024-10-302024-10-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/178560eng2041-1723PURE: 107717056https://doi.org/10.1038/s41467-024-53699-zinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-02-24T01:40:36Zoai:run.unl.pt:10362/178560Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T19:46:53.931608Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Catalytic peptide-based coacervates for enhanced function through structural organization and substrate specificity
title Catalytic peptide-based coacervates for enhanced function through structural organization and substrate specificity
spellingShingle Catalytic peptide-based coacervates for enhanced function through structural organization and substrate specificity
Reis, David Q.P.
Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
title_short Catalytic peptide-based coacervates for enhanced function through structural organization and substrate specificity
title_full Catalytic peptide-based coacervates for enhanced function through structural organization and substrate specificity
title_fullStr Catalytic peptide-based coacervates for enhanced function through structural organization and substrate specificity
title_full_unstemmed Catalytic peptide-based coacervates for enhanced function through structural organization and substrate specificity
title_sort Catalytic peptide-based coacervates for enhanced function through structural organization and substrate specificity
author Reis, David Q.P.
author_facet Reis, David Q.P.
Pereira, Sara
Ramos, Ana P.
Pereira, Pedro M.
Morgado, Leonor
Calvário, Joana
Henriques, Adriano O.
Serrano, Mónica
Pina, Ana S.
author_role author
author2 Pereira, Sara
Ramos, Ana P.
Pereira, Pedro M.
Morgado, Leonor
Calvário, Joana
Henriques, Adriano O.
Serrano, Mónica
Pina, Ana S.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
UCIBIO - Applied Molecular Biosciences Unit
Faculdade de Ciências e Tecnologia (FCT)
RUN
dc.contributor.author.fl_str_mv Reis, David Q.P.
Pereira, Sara
Ramos, Ana P.
Pereira, Pedro M.
Morgado, Leonor
Calvário, Joana
Henriques, Adriano O.
Serrano, Mónica
Pina, Ana S.
dc.subject.por.fl_str_mv Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
topic Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)
description This work was partially supported by PPBI - Portuguese Platform of BioImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE - \u201COr\u00E7amento de Estado\u201D and by european funds from FEDER - \u201CFundo Europeu de Desenvolvimento Regional. La Caixa Junior Leader Fellowship (LCF/BQ/PI20/11760012) financed by\u201Dla Caixa\u201D Foundation (ID 100010434) and a Maratona da Sa\u00FAde award. The NMR spectrometers at CERMAX, ITQB-NOVA, Oeiras are funded by FCT through project AAC 01/SAICT/2016, while those from FCT-NOVA are part of the National NMR Network and are supported by FCT (ROTEIRO/0031/2013) cofounded by FEDER through COMPETE 2020, POCI, PORL and FCT through PIDDAC. Publisher Copyright: © The Author(s) 2024.
publishDate 2024
dc.date.none.fl_str_mv 2024-10-30
2024-10-30T00:00:00Z
2025-02-06T21:19:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/178560
url http://hdl.handle.net/10362/178560
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2041-1723
PURE: 107717056
https://doi.org/10.1038/s41467-024-53699-z
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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