Genetic instability of a commercial Saccharomyces cerevisiae strain

Detalhes bibliográficos
Autor(a) principal: Schuller, Dorit Elisabeth
Data de Publicação: 2005
Outros Autores: Alves, Hugo, Cambon, Brigitte, Dequin, Sylvie, Casal, Margarida
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/1822/3366
Resumo: Resumo e poster apresentados no XXII International Conference on Yeast Genetics and Molecular Biology, Bratislava, Eslováquia
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spelling Genetic instability of a commercial Saccharomyces cerevisiae strainInterdelta sequence typingMitochondrial DNA RFLPKaryotypingMicrosatellite analysisCommercial yeastResumo e poster apresentados no XXII International Conference on Yeast Genetics and Molecular Biology, Bratislava, EslováquiaThe usefulness of four genetic fingerprinting methods (interdelta sequence typing, mitochondrial DNA restriction length polymorphism (mtDNA RFLP), chromosomal karyotyping and microsatellite analysis) was assessed in order to detect a commercial yeast strain (Zymaflore VL1, Lallemand). From 54 spontaneous fermentations performed with grapes collected in 18 the Vinho Verde Wine Region in northwest Portugal, 101 isolates were recovered whose mtDNA RFLP were identical to strain Zymaflore VL1. Evaluation of polymorphisms was performed in comparison to 30 isolates of the corresponding original commercialized strain. Microsatellite allelic polymorphisms were found in 12 natural isolates, two of them were characterized by complete loss of heterozygosity, whereas their chromosomal constitution showed a loss of structural heteromorphism. Major changes of chromosomal patterns were found among the natural isolates, apparent by the absence or changed position of bands in the presumable region of chromosomes VI and III. Interdelta amplification patterns depended on the primer pair used, and changes were apparent by additional bands. No correlations were found between interdelta sequence amplification patterns and chromosomal profiles or microsatellite typing patterns. The present data show that commercial yeast strains present a considerable genetic instability that can be assessed by distinct methods. Whether these changes are associated with the yeast’s permanence in natural environments is subject of current investigations.União Europeia - Fundo Social Europeu (FSE) – III Quadro Comunitário de Apoio (QCA III) - Programa Operacional Agricultura e Desenvolvimento Rural (AGRO) - AGRO, medida 8 - EnoSafe Nº 762.União Europeia (U.E.) - III Quadro Comunitário de Apoio (QCA III) - Programa Operacional Ciência e Inovação 2010 (POCI 2010) - project POCI/AGR/56771/2004.Universidade do MinhoSchuller, Dorit ElisabethAlves, HugoCambon, BrigitteDequin, SylvieCasal, Margarida2005-082005-08-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/1822/3366enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T06:41:53Zoai:repositorium.sdum.uminho.pt:1822/3366Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:01:43.850272Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Genetic instability of a commercial Saccharomyces cerevisiae strain
title Genetic instability of a commercial Saccharomyces cerevisiae strain
spellingShingle Genetic instability of a commercial Saccharomyces cerevisiae strain
Schuller, Dorit Elisabeth
Interdelta sequence typing
Mitochondrial DNA RFLP
Karyotyping
Microsatellite analysis
Commercial yeast
title_short Genetic instability of a commercial Saccharomyces cerevisiae strain
title_full Genetic instability of a commercial Saccharomyces cerevisiae strain
title_fullStr Genetic instability of a commercial Saccharomyces cerevisiae strain
title_full_unstemmed Genetic instability of a commercial Saccharomyces cerevisiae strain
title_sort Genetic instability of a commercial Saccharomyces cerevisiae strain
author Schuller, Dorit Elisabeth
author_facet Schuller, Dorit Elisabeth
Alves, Hugo
Cambon, Brigitte
Dequin, Sylvie
Casal, Margarida
author_role author
author2 Alves, Hugo
Cambon, Brigitte
Dequin, Sylvie
Casal, Margarida
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Schuller, Dorit Elisabeth
Alves, Hugo
Cambon, Brigitte
Dequin, Sylvie
Casal, Margarida
dc.subject.por.fl_str_mv Interdelta sequence typing
Mitochondrial DNA RFLP
Karyotyping
Microsatellite analysis
Commercial yeast
topic Interdelta sequence typing
Mitochondrial DNA RFLP
Karyotyping
Microsatellite analysis
Commercial yeast
description Resumo e poster apresentados no XXII International Conference on Yeast Genetics and Molecular Biology, Bratislava, Eslováquia
publishDate 2005
dc.date.none.fl_str_mv 2005-08
2005-08-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/3366
url http://hdl.handle.net/1822/3366
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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