Genetic instability of a commercial Saccharomyces cerevisiae strain

Bibliographic Details
Main Author: Schuller, Dorit Elisabeth
Publication Date: 2006
Other Authors: Alves, Hugo, Cambon, Brigitte, Dequin, Sylvie, Casal, Margarida
Language: por
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/5078
Summary: Resumo e slides da comunicação oral apresentada no encontro científico "XXXII Jornadas Portuguesas de Genética" Vila Real, Portugal, 2006.
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spelling Genetic instability of a commercial Saccharomyces cerevisiae strainGenetic fingerprintingInterdelta sequence typingMitochondrial DNA restriction length polymorphismChromosomal karyotypingSpontaneous fermentationsLoss of heterozygosityStructural heteromorphismZymaflore VL1Genetic instabilityResumo e slides da comunicação oral apresentada no encontro científico "XXXII Jornadas Portuguesas de Genética" Vila Real, Portugal, 2006.The usefulness of four genetic fingerprinting methods (interdelta sequence typing, mitochondrial DNA restriction length polymorphism (mtDNA RFLP), chromosomal karyotyping and microsatellite analysis) was assessed in order to detect a commercial yeast strain (Zymaflore VL1, Lallemand). From 54 spontaneous fermentations performed with grapes collected in 18 the Vinho Verde Wine Region in northwest Portugal, 101 isolates were recovered whose mtDNA RFLP were identical to strain Zymaflore VL1. Evaluation of polymorphisms was performed in comparison to 30 isolates of the corresponding original commercialized strain. Microsatellite allelic polymorphisms were found in 12 natural isolates, two of them were characterized by complete loss of heterozygosity, whereas their chromosomal constitution showed a loss of structural heteromorphism. Major changes of chromosomal patterns were found among the natural isolates, apparent by the absence or changed position of bands in the presumable region of chromosomes VI and III. Interdelta amplification patterns depended on the primer pair used, and changes were apparent by additional bands. No correlations were found between interdelta sequence amplification patterns and chromosomal profiles or microsatellite typing patterns. The present data show that commercial yeast strains present a considerable genetic instability that can be assessed by distinct methods. Whether these changes are associated with the yeast’s permanence in natural environments is subject of current investigations.Este estudo foi desenvolvido no âmbito do projecto ENOSAFE (Nº 762, Programa AGRO, medida 8) Instituto Nacional de Investigação Agrária and POCI 2010 (POCI/AGR/56102/2004) Fundação para a Ciência e Tecnologia, Portugal (FCT)Universidade do MinhoSchuller, Dorit ElisabethAlves, HugoCambon, BrigitteDequin, SylvieCasal, Margarida2006-042006-04-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/1822/5078porinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T07:06:10Zoai:repositorium.sdum.uminho.pt:1822/5078Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:15:23.332985Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Genetic instability of a commercial Saccharomyces cerevisiae strain
title Genetic instability of a commercial Saccharomyces cerevisiae strain
spellingShingle Genetic instability of a commercial Saccharomyces cerevisiae strain
Schuller, Dorit Elisabeth
Genetic fingerprinting
Interdelta sequence typing
Mitochondrial DNA restriction length polymorphism
Chromosomal karyotyping
Spontaneous fermentations
Loss of heterozygosity
Structural heteromorphism
Zymaflore VL1
Genetic instability
title_short Genetic instability of a commercial Saccharomyces cerevisiae strain
title_full Genetic instability of a commercial Saccharomyces cerevisiae strain
title_fullStr Genetic instability of a commercial Saccharomyces cerevisiae strain
title_full_unstemmed Genetic instability of a commercial Saccharomyces cerevisiae strain
title_sort Genetic instability of a commercial Saccharomyces cerevisiae strain
author Schuller, Dorit Elisabeth
author_facet Schuller, Dorit Elisabeth
Alves, Hugo
Cambon, Brigitte
Dequin, Sylvie
Casal, Margarida
author_role author
author2 Alves, Hugo
Cambon, Brigitte
Dequin, Sylvie
Casal, Margarida
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Schuller, Dorit Elisabeth
Alves, Hugo
Cambon, Brigitte
Dequin, Sylvie
Casal, Margarida
dc.subject.por.fl_str_mv Genetic fingerprinting
Interdelta sequence typing
Mitochondrial DNA restriction length polymorphism
Chromosomal karyotyping
Spontaneous fermentations
Loss of heterozygosity
Structural heteromorphism
Zymaflore VL1
Genetic instability
topic Genetic fingerprinting
Interdelta sequence typing
Mitochondrial DNA restriction length polymorphism
Chromosomal karyotyping
Spontaneous fermentations
Loss of heterozygosity
Structural heteromorphism
Zymaflore VL1
Genetic instability
description Resumo e slides da comunicação oral apresentada no encontro científico "XXXII Jornadas Portuguesas de Genética" Vila Real, Portugal, 2006.
publishDate 2006
dc.date.none.fl_str_mv 2006-04
2006-04-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/5078
url http://hdl.handle.net/1822/5078
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833595837261807616

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