Survey of molecular methods for the typing of wine yeast strains

Bibliographic Details
Main Author: Schuller, Dorit Elisabeth
Publication Date: 2004
Other Authors: Valero, Eva, Dequin, Sylvie, Casal, Margarida
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://hdl.handle.net/1822/2213
Summary: A survey of the genetic polymorphisms produced by distinct methods was performed in 23 commercial winery yeast strains. The microsatellite typing, using 6 different loci, an optimized interdelta sequence analysis and RFLP of mitochondrial DNA generated by the enzyme Hinf I had the same discriminatory power: among the 23 commercial yeast strains, 21 distinct patterns were obtained. Karyotype analysis originated 22 patterns, thereby allowing the discrimination of one of the three strains that were not distinguished by the other methods. Due to the equivalence of the results obtained in this survey, any of the methods can be applied at the industrial scale.
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spelling Survey of molecular methods for the typing of wine yeast strainsMolecular typingKaryotypingInterdelta sequenceMitochondrial DNA RFLPMicrosatelliteCommercial yeastmitochondrial DNA restriction fragment length polymorphismScience & TechnologyA survey of the genetic polymorphisms produced by distinct methods was performed in 23 commercial winery yeast strains. The microsatellite typing, using 6 different loci, an optimized interdelta sequence analysis and RFLP of mitochondrial DNA generated by the enzyme Hinf I had the same discriminatory power: among the 23 commercial yeast strains, 21 distinct patterns were obtained. Karyotype analysis originated 22 patterns, thereby allowing the discrimination of one of the three strains that were not distinguished by the other methods. Due to the equivalence of the results obtained in this survey, any of the methods can be applied at the industrial scale.Fundação para a Ciência e a Tecnologia (FCT) - POCTI/BIO/38106/2001 (Eixo 2, Medida 2.3, QCAIII - FEDER).Instituto de Cooperação Científica e Tecnológica Internacional (ICCTI) - grant nº 657 C2.Comunidade Europeia (UE) - contract number QLK4-CT-2001-51873.Elsevier B.V.Universidade do MinhoSchuller, Dorit ElisabethValero, EvaDequin, SylvieCasal, Margarida20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/2213eng"FEMS microbiology letters". ISSN 0378-1097. 231:1 (2004) 19-26.0378-109710.1016/S0378-1097(03)00928-514769461http://www.elsevier.com/wps/find/journaldescription.cws_home/506058/description#descriptioninfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-04-12T05:02:10Zoai:repositorium.sdum.uminho.pt:1822/2213Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:56:11.377100Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Survey of molecular methods for the typing of wine yeast strains
title Survey of molecular methods for the typing of wine yeast strains
spellingShingle Survey of molecular methods for the typing of wine yeast strains
Schuller, Dorit Elisabeth
Molecular typing
Karyotyping
Interdelta sequence
Mitochondrial DNA RFLP
Microsatellite
Commercial yeast
mitochondrial DNA restriction fragment length polymorphism
Science & Technology
title_short Survey of molecular methods for the typing of wine yeast strains
title_full Survey of molecular methods for the typing of wine yeast strains
title_fullStr Survey of molecular methods for the typing of wine yeast strains
title_full_unstemmed Survey of molecular methods for the typing of wine yeast strains
title_sort Survey of molecular methods for the typing of wine yeast strains
author Schuller, Dorit Elisabeth
author_facet Schuller, Dorit Elisabeth
Valero, Eva
Dequin, Sylvie
Casal, Margarida
author_role author
author2 Valero, Eva
Dequin, Sylvie
Casal, Margarida
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Schuller, Dorit Elisabeth
Valero, Eva
Dequin, Sylvie
Casal, Margarida
dc.subject.por.fl_str_mv Molecular typing
Karyotyping
Interdelta sequence
Mitochondrial DNA RFLP
Microsatellite
Commercial yeast
mitochondrial DNA restriction fragment length polymorphism
Science & Technology
topic Molecular typing
Karyotyping
Interdelta sequence
Mitochondrial DNA RFLP
Microsatellite
Commercial yeast
mitochondrial DNA restriction fragment length polymorphism
Science & Technology
description A survey of the genetic polymorphisms produced by distinct methods was performed in 23 commercial winery yeast strains. The microsatellite typing, using 6 different loci, an optimized interdelta sequence analysis and RFLP of mitochondrial DNA generated by the enzyme Hinf I had the same discriminatory power: among the 23 commercial yeast strains, 21 distinct patterns were obtained. Karyotype analysis originated 22 patterns, thereby allowing the discrimination of one of the three strains that were not distinguished by the other methods. Due to the equivalence of the results obtained in this survey, any of the methods can be applied at the industrial scale.
publishDate 2004
dc.date.none.fl_str_mv 2004
2004-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/2213
url https://hdl.handle.net/1822/2213
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "FEMS microbiology letters". ISSN 0378-1097. 231:1 (2004) 19-26.
0378-1097
10.1016/S0378-1097(03)00928-5
14769461
http://www.elsevier.com/wps/find/journaldescription.cws_home/506058/description#description
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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