Atividade antimicrobiana de extratos hidroetanólicos de joannesia princeps (euphorbiaceae)

• Ano de defesa: 2018
• Autor(a) principal: Costa, Maria Silvana Totti da
• Orientador(a): Weckwerth, Paulo Henrique
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Sagrado Coração
• Programa de Pós-Graduação: Biologia Oral
• Departamento: Ciências da Saúde e Biológicas
• País: Brasil
• Palavras-chave em Português: Produtos naturais; Joannesia princeps; Atividade antimicrobiana; Citotoxicidade
• Palavras-chave em Inglês: Natural products; Joannesia princeps; Antimicrobian activity; Cytotoxicity
• Área do conhecimento CNPq: CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://tede2.usc.br:8443/handle/tede/458

Evidence of the antimicrobial activity of innumerable natural products and the need for economically acceptable and potent drugs against microorganisms resistant to synthetic antibiotics are required. The use of herbal medicine is millenarian and the use of medicinal plants to treat diseases has been little explored. The plant Joannesia princeps (Euphorbiaceae) presents many biological activities, being its seeds used in popular medicine like purgative. The objective of this study was to investigate the antimicrobial activity of hydrotheranic extracts of leaves, stem and seeds of J. princeps on different strains of microorganisms, as well as to develop laboratory methodologies used for the evaluation of antimicrobial activity. Antimicrobial activity, the inhibition of biofilm formation by candidas and the cytotoxicity of extracts in cell culture were determined. The extract of the leaves of J. princeps presented antimicrobial activity for 12 of the 21 strains tested and was effective against three isolated yeasts. The extract of the stem of J. princeps presented antimicrobial activity only against M luteus (ATCC 10240), whereas the extract of the seeds showed absence of activity on the microorganisms tested. Extracts of J. princeps did not inhibit biofilm formation by any single Candida species. The tests showed toxicity of the extracts against BHK-21 cells. This study showed an important antimicrobial activity of leaf extracts of J. princeps even at a maximum concentration of 12.5 mg / mL, so it is worthwhile to consider other studies using higher concentrations.