Caracterização do polimorfismo da base (-251) do gene da Interleucina 8 em pacientes com gastrite crônica

• Ano de defesa: 2010
• Autor(a) principal: Fabris, Rita de Cássia
• Orientador(a): Payão, Spencer Luiz Marques
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: IASCJ - Universidade Sagrado Coração
• Programa de Pós-Graduação: Biologia Oral
• Departamento: Ciências da Saúde e Biológicas
• País: BR
• Palavras-chave em Português: HELICOBACTER PYLORI; GENOTIPAGEM; INTERLEUCINA 8; PACIENTES COM GASTRITE CRÔNICA
• Palavras-chave em Inglês: HELICOBACTER PYLORI; GENOTYPING; INTERLEUKIN 8; PATIENTS WITH CHRONIC GASTRITIS
• Área do conhecimento CNPq: CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: http://localhost:8080/tede/handle/tede/118

It is estimated that at least half of the human population is infected by Helicobacter pylori. It is an important pathogen associated with the development of chronic gastritis, peptic ulcer disease and gastric cancer. Several authors reported that the infiltration of inflammatory cells, including neutrophils and mononuclear cells is the trace of the pathology of gastric mucosa, associated with the infection. There is high evidence that inflammation is associated with severity of gastric lesions.Interleukin-8 (IL-8), a member of cytokines family, is an activator and chemoattractant of neutrophils and lymphocytes. It has been reported that increased gastric mucosal levels of IL-8 is associated with H. pylori infection. The objectives of this paper were (i) characterize Interleukin-8 -251T>A polymorphism and (ii) to examine the possible relationship between this polymorphism and the H. pylori infection. One hundred and sixty patients, (with a mean age 48.7 years) presenting recurrent abdominal pain participated in the study: 107 H. pylori positives and 53 H. pylori negatives previously diagnosed by PCR. IL8 -251T>A genotypes were determined using a polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) in all samples. Our findings indicate that there is no association of IL-8 -251T>A with H. pylori-infected patients or gender of these patients and these conclusions were consistent with other reports from different population samples.