Detalhes bibliográficos
| Ano de defesa: |
2013 |
| Autor(a) principal: |
BATISTA, Jacqueline Ellen Camelo
 |
| Orientador(a): |
LIMA FILHO, José Vitor Moreira |
| Banca de defesa: |
PEIXOTO, Silvio Ricardo Maurano,
MENDES, Emiko Shinozaki,
SHINOHARA, Neide Kazue Sakugawa |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal Rural de Pernambuco
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciência Animal Tropical
|
| Departamento: |
Departamento de Morfologia e Fisiologia Animal
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5052
|
Resumo: |
The species Anomalocardia brasiliana is found in abundance on the beaches of the Brazilian coast and much appreciated in Northeastern cuisine. This study aimed to characterize molecular enterotoxins and resistance gene mecA in Staphylococcus spp. isolates of coagulase-negative beef samples A. brasiliana in the metropolitan area of Recife, Brazil. Isolated from fresh samples (n = 9), collected in the resort of Mangue Seco, Igarassu-PE, were identified as Staphylococcus xylosus (4/9), Staphylococcus cohnii spp. urealyticus (4/9) and Staphylococcus sciuri (1/9). Among commercialized samples (n = 21) were identified Staphylococcus sciuri (16/21), Staphylococcus xylosus (4/21) and Staphylococcus lentus (1/21). The determination of antibiotic resistance profile was performed following the CLSI guidelines. The highest rates of resistance were observed in isolates from fresh samples against erythromycin (58.53%), penicillin (51.21%) and tetracycline (43.9%). Isolated from samples marketed presented greater resistance to oxacillin (55.3%) and penicillin (36.8%). All isolates were characterized as coagulase-negative and were resistant to oxacillin and / or cefoxitin and positive for the presence of mecA gene but phenotypically susceptible to vancomycin. Also, the genes for enterotoxins seg and seh were detected in 77,7% and 88,8% of isolates from environmental samples, versus 90.5% and 100% of the isolates of real samples, respectively. The results of this study reveal the presence of Staphylococcus coagulase-negative methicillin-resistant carriers of toxigenic genes in bivalve mollusk A. brasiliana. This is first report of Staphylococcus coagulase-negative methicillin-resistant bivalve mollusk in A. brasiliana. |
