Detalhes bibliográficos
| Ano de defesa: |
2009 |
| Autor(a) principal: |
COSTA, Nivaldo de Azevêdo
 |
| Orientador(a): |
MENDONÇA, Carla Lopes de |
| Banca de defesa: |
SILVA, José Augusto Bastos Afonso da,
SENA, Maria José de,
SOARES, Pierre Castro,
BARBOSA NETO, Jose Diomedes,
SOUZA, Jose Claudio de Almeida |
| Tipo de documento: |
Tese
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal Rural de Pernambuco
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciência Veterinária
|
| Departamento: |
Departamento de Medicina Veterinária
|
| País: |
Brasil
|
| Palavras-chave em Português: |
|
| Palavras-chave em Inglês: |
|
| Área do conhecimento CNPq: |
|
| Link de acesso: |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5801
|
Resumo: |
The aim of the present study was to evaluate the alterations on the protein screen and serum cupper, iron and zinc levels of Santa Inês primiparous ewes with mastitis experimentally induced with Staphylococcus aureus. The right mammary gland of ten healthy ewes was inoculated with 1,0x104 ufc/mL. The clinical exam; eletrophoresis profile in poliacrilamide gel (SDS-PAGE), concentration of serum cupper, iron and zinc and plasma fibrinogen were measured before the inoculation (baseline moment) and in the following moments: 12, 24, 36, 48, 60, 72, 84, 96, 108, 120, 132, 168, 180, 288 and 336 hours after the inoculation of the agent. All animals experimentally infected presented clinical mastitis and subsequent loss of functionality of the gland. The protein screen showed 23 proteins fractions with molecular weights (MW) varying from 26.000 to 185.000 daltons (Da) allowing the recognition of positive and negative acute-phase proteins, class A and G immunoglobulin’s and some non-identified proteins. A significant increase (P<0,05) in haptoglobin and ceruloplasmin concentration, IgG and IgA was observed. Antitrypsin and acid glicoprotein concentrations (P<0,05) did not alter. The average levels of iron and zinc decreased (P<0,05)and the cupper values increased (P<0,05). A positive correlation between plasma fibrinogen and ceruloplasmin (r=0,74), haptoglobin (r=0,62) and IgA (r=0,62) was also identified. Results showed the importance of ceruloplasmin and haptoglobin as acute-phase proteins in intramammary infections of ewes and reiterate fibrinogen as an inflammatory marker because of its high correlation with specific proteins. The serum alterations of Cu, Fe and Zn revealed the action of inflammatory mediators triggered by S. aureus. |
