Proteinograma do soro sanguíneo e lácteo de ovelhas da raça Santa Inês em diferentes fases da lactação

• Ano de defesa: 2013
• Autor(a) principal: LEMOS, Vânia Freire
• Orientador(a): MENEZES, Carla Lopes de
• Banca de defesa: AFONSO, José Augusto Bastos, SOARES, Pierre Castro, FIORAVANTI, Maria Clorinda Soares
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal Rural de Pernambuco
• Programa de Pós-Graduação: Programa de Pós-Graduação em Sanidade e Reprodução de Ruminantes
• Departamento: Unidade Acadêmica de Garanhuns
• País: Brasil
• Palavras-chave em Português: Infecção intramamária; Lactação; Ovelhas; Proteína sérica; Proteína láctea; Blood serum; Intramamary infection; Lactation, Ewes; Serum protein; Whey protein
• Área do conhecimento CNPq: MEDICINA VETERINARIA::REPRODUCAO ANIMAL
• Link de acesso: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6303

The objective of this work was to evaluate dynamics of the proteinogram of blood serum and whey protein of Santa Ines sheep breed following the antipartum period and during lactation, and to compare/to quantify proteins detected at eletrophoresis of the whey protein from healthy and infectious mammary glands in different phases of lactation. Thrirty four sheeps submitted to half-intensive system with same sanitary and nutritional management has been followed. For accomplishment of proteinogram of the sheep, they had been investigated during approximately 10 days from antipartum and 15, 30, 60 and 90 days postpartum, moments where clinical examination of mammary gland was carried through. Blood serum was evaluated at antipartum moment and proteinogram of whey protein at subsequent moments. Bacteriological culture and biochemist characterization of milk samples for confirmation of healthy and infected glands was performed. Separation of protein fractions was carried through using sodium dodecil sulphate polyacrylamide gel eletrophoresis (SDS-PAGE). For the blood serum it was observed quantification of nine proteins with significant influence in IgG. At whey protein, influence of the phases of lactation was identified eigth proteins, having albumin, IgG and β - lactoglobulin.. Comparing healthy and infected glands it was verified that hatptoglobin and α-1 acid glycoprotein, lactoferrin, albumin and immunoglobulins IgA and IgG in whey protein act as potentials biomarkers of infection in mammary gland of ovine species.