Detalhes bibliográficos
| Ano de defesa: |
2013 |
| Autor(a) principal: |
LIMA, Elizabeth Hortêncio Ferreira
 |
| Orientador(a): |
SILVA, José Augusto Bastos Afonso da |
| Banca de defesa: |
MIRANDA NETO, Eldinê Gomes de,
SOARES, Pierre Castro,
MENDONÇA , Carla Lopes de |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal Rural de Pernambuco
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Sanidade e Reprodução de Ruminantes
|
| Departamento: |
Unidade Acadêmica de Garanhuns
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6238
|
Resumo: |
This study aimed to investigate the effect of administration of sodium monensin in the diet on blood metabolites and rumen of ewes before, during and pospartum, due to the complexity that this period is due to anatomical changes, physiological and metabolic changes in female to meet the higher demand for energy growth of the fetus and lactation. A prospective study was conducted involving 13 sheep, in order to evaluate the effect of monensin supplemented from 60 days before calving (dap), on the hormonal and metabolic profile in periods of antepartum and postpartum. We used 13 Santa Inês sheep, pregnant, clinically healthy. Two groups were formed randomly, a control group receiving bulky, balanced feed and mineral salt and another group that received beyond bulky, balanced ration containing 30 mg monensin / day and mineral salt with monensin. Blood and ruminal fluid were collected at 60, 50, 40, 30, 20, 10 days before delivery, at birth and at 10, 20 and 30 days postpartum. We conducted the CBC and biochemical measurement of the following variables: glucose, non-esterified fatty acids (NEFA), β-hydroxybutyrate, cholesterol, triglycerides, fructosamine, total protein, albumin, urea, creatinine and research ketone bodies in urine. Enzyme activity by aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), creatine kinase (CK) and hormonal determinations of cortisol and insulin. In the analysis of ruminal fluid pH were determined, proof of reduction of methylene blue (PRAM), the infusoria, the chloride content and the concentration of volatile fatty acids (VFA) (acetic, propionic and butyric). Statistical analysis was employed ANOVA and correlation study (P<0,05). With regard to haematological values in different physiological periods studied, there was no significant difference (P> 0,05), except for the white blood count revealed a mild leukocytosis with neutrophilia at birth significantly (P <0,05) in two groups. The enzymatic activity of AST levels were more elevated in the control group (P<0,05) during the postpartum period and significant differences were also recorded between groups, no 10 dpp and 20 dpp (P<0,05) and higher in the control group compared to monensin. There was no difference in cholesterol (P>0,05). Effect of time (P<0,05) was observed with respect to triglycerides in both groups, being higher in the pre-partum. Monensin significantly (P<0,05) increased concentration of propionate in the rumen, fructosamine and insulin. Little effect was observed for glucose, NEFA and BHB. No treatment effect (P>0,05) was observed for pH, chloride and diatomaceous the rumen, and the protein profile. The administration of monensin in ewe in the period before the birth did not influence the indicators hematologic and enzymatic activity, but caused improvement in some indicators of energy balance, this result is considered important for the prevention of metabolic diseases associated with energy deficit that occurs this period, such as toxemia of pregnancy. |
