Identificação de SNPs para uma anomalia de desenvolvimento em E. grandis e seus efeitos na estrutura proteica e interatoma

Detalhes bibliográficos
Ano de defesa: 2015
Autor(a) principal: Martin, Leonardo Curi [UNESP]
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual Paulista (Unesp)
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://hdl.handle.net/11449/132200
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/02-10-2015/000851181.pdf
Resumo: Eucalyptus species have been successfully used for forest plantations. Some intrinsic features such as rapid growth, adaptability to various weather conditions and economic potential in energy production, fiber and solid wood, help to reduce pressure on tropical forests and associated biodiversity. Investments in research and development on molecular biology arebeing carried out by forestry sector in order to increase productivity gains. Since anomalous characteristics of progeny may generate productivity loss, gene identification linked to abnormalities are crucial for breeding programs. During a controlled crossing with Eucalyptus grandis, the Suzano Papel e Celulose enterprise detected an atypical Mendelian segregation 3: 1 (normal: abnormal). Significant phenotypic differences concerning height, stem base diameter, and leaf size appeared, as well as an unusual early death, when compared to normal plants. Previous studies identified a molecular tag attached to this anomaly. Sequence comparison analysis of eucalyptus database showed identity with Bet v1 PR10 protein family (Pathogenesis-related protein 10). However, gene involvement in developing such aberration is undetermined. Other studies found a transcriptional profile of contrasting phenotypes by RNAseq technique. Among differentially expressed genes by functional enrichment highlighted those belonging to Bet v1 families, chitinases class I, and thaumatin, all related to pathogenic response. This fact suggests that aberration is caused by the plant immune system activation (autoimmune response). To investigate the pathogenesis of such genetic disorder, three approaches were adopted: (i) SNPs identification among the differentially expressed genes of Bet v1 families, chitinase, and thaumatin, (ii) miRNAs identification with potential binding to mRNAs differentially expressed genes, and (iii) check of possible influence of differentially expressed gene products on ...