Matrizees tridimensionais a base de poli (3 hidroxibutirato) produzidas por sinterização seletiva a laser e funcionalizadas com fibroína da seda, hidroxiapatita e peptídeo osteogênico. Avaliações físico-química e biológica

• Ano de defesa: 2015
• Autor(a) principal: Pires, Luana Carla [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Engenharia tecidual; Polímeros; Apatita; Tissue engineering
• Link de acesso: http://hdl.handle.net/11449/145494

Three-dimensional structures (scaffolds) fabricated by three-dimensional printing techniques (3DP), have been widely used in tissues reconstruction and regeneration. The main advantage of that technique is the possibility of manufacturing scaffolds with complex shapes. The aim of this study was to perform physicochemical and biological characterization of three-dimensional matrices of poly (3-hydroxybutyrate) (PHB), made by 3DP via selective laser sintering (SLS), functionalized with silk fibroin (FB), apatites (HA) and / or osteogenic growth peptide (OGP), to favor bone regeneration. The results demonstrated that SLS 3DP produced PHB scaffolds with porosity percentage of 55.8 ± 0.7% with regular pores of 500-700 µm. The addition of HA and FB results in similar porosity and enhanced PHB water absorption rate, at the 2h timepoint. The incorporation of FB improved compressive strength and elastic modulus, which was also improved by FB-HA. In vitro tests showed good cell adhesion, spreading and proliferation in all. In the indirect evaluation of calcium precipitation a better result was observed in FB-HA group mainly in 3 days. In vivo experimental groups failed to stimulate contact osteogenesis. However, the scaffolds preserved the thickness of implantation area, which did not occur in the control clot group. It was concluded that the scaffolds evaluated in this study have some favorable properties for bone regeneration. However some of their action mechanisms in vivo, especially in long-term periods, still have to be better understood.