Ocorrência, sorotipagem e rsistência antimicrobiana de Streptococcus suis em funcionários e ambente de abatedouro de sínos

• Ano de defesa: 2014
• Autor(a) principal: Soares, Taíssa Cook Siqueira [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Estreptococo; Epidemiologia - Pesquisa; Agentes anti-inflamatorios; Trabalhadores - Fatores de risco; Matadouros; Slaughtering and slaughter-houses
• Link de acesso: http://hdl.handle.net/11449/123291; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/08-05-2015/000829410.pdf

The present study aimed to determine the occurrence of Streptococcus suis in employees (healthy carriers) and environment of swine slaughterhouses with federal inspection service of the state of São Paulo, Brazil, to describe the distribution of the different types of the agent and the antimicrobial susceptibility profile of the isolated strains, as well as to research the risk factors associated with the occurrence of the pathogen. Tonsillar swabs of 139 employees and 261 environmental swabs were collected for detection of S. suis and its types by monoplex and multiplex PCR, respectively. There was no detection of healthy carriers employees. S. suis was isolated from 27.03% of the samples collected at slaughterhouse A; 17.31% at slaughterhouse B; 15.31% at slaughterhouse C; and 36.36% at slaughterhouse D. Significant statistical differences (p<0.05) were observed for the occurrence of S. suis among slaughterhouses and among areas of low, medium and high risk of the slaughterhouse A. The types 4 (20.00%), 5 (20.00%), 12 (20.00%) and 29 (20.00%) were the most frequent followed by types 21 (10.00%) and 31 (10.00%). The most effective antimicrobials were amoxicillin-clavulanate (100.00%), doxycycline (100.00%), ceftiofur (93.75%), ampicillin (81.25%) and cephalexin (75.00%). Multidrug resistance was observed in 100.00% of the isolates. The slaughterhouses and the different areas of the pig slaughtering may be risk factors for the occurrence of S. suis and, consequently, for human infection. However, it is noteworthy that all employees are exposed to S. suis infection. This project is the first epidemiological study in Brazil about the risk factors associated with the occurrence of S. suis involving a large numbers of individuals belonging to the main risk group