Influência de diferentes métodos de esterilização de esmalte dental sobre a sua morfologia, composição química, estrutura e formação de biofilmes in vitro

• Ano de defesa: 2013
• Autor(a) principal: Viana, Patricia Gabriela Sabino [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Esmalte dentário; Esterilização; Biofilme; Dental enamel
• Link de acesso: http://hdl.handle.net/11449/127543; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/25-08-2015/000839097.pdf

The sterilization process should preserve the enamel properties of specimens used for in vitro and in situ studies. The aim of this study was to evaluate the influence of sterilization methods Microwaving - MI, Gamma irradiation - GI, Ethylene oxide - EO and Steam autoclave SA on the enamel properties. This study was conducted into 2 phases. For phase 1, 50 specimens of bovine enamel were assigned into 5 groups (n=10): MI, GI, EO, SA and control (no sterilization), evaluated regarding morphology (Scanning Electron Microscopy - SEM superficial and cross-section), chemical composition (X-ray energy dispersive spectroscopy - EDS and X-ray photoelectron spectroscopy - XPS) and structure (Raman and Nuclear Magnetic Resonance - RMN). For phase 2, 360 bovine enamel specimens were assigned into 4 groups (n=90): MI, GI, EO and SA. For each group, biofilms were grown as monospécies (Actinomyces naeslundii and Streptococcus sanguis) and multispecies, evaluated regarding counting forming units (CFU/mL), metabolic activity (reduction of 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide - XTT) and biomass (violet crystal assay). Statistical evaluation was performed for data from XPS, Raman, CFU/mL, XTT assay and violet crystal assay. The results indicated that GI group produced no alterations on the morphology, chemical composition and structure of the enamel. MI and EO groups promoted slightly alterations on morphology, chemical composition and structure. The SA group caused severe morphological changes on surface and in depth and small alterations on chemical composition and structure. There were no differences among groups for S. sanguis biofilms. A. naeslundii biofilms showed higher values of CFU/mL and biomass for SA group and higher metabolic activity in GI group. It can be concluded that enamel properties could be influenced ...(Complete abstract electronic access below)