Efeitos de defensivos agrícolas naturais e extratos vegetais sobre parâmetros biológicos de Metarhizium anisopliae (Metsch.) Sorok

• Ano de defesa: 2011
• Autor(a) principal: Mamprim, Ana Paula
• Orientador(a): Alves, Luis Francisco Angeli
• Banca de defesa: Stangarlin, José Renato , Almeida, José Eduardo Marcondes de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual do Oeste do Paraná; Marechal Cândido Rondon
• Programa de Pós-Graduação: Programa de Pós-Graduação em Agronomia
• Departamento: Centro de Ciências Agrárias
• País: BR
• Palavras-chave em Português: Fungos entomopatogênicos; Compatibilidade; Extratos vegetais; Produtos alternativos
• Palavras-chave em Inglês: Entomopathogenic fungi; Compatibility; Plant extracts; Alternative products
• Área do conhecimento CNPq: CIÊNCIAS AGRÁRIAS:AGRONOMIA
• Link de acesso: http://tede.unioeste.br:8080/tede/handle/tede/1420

The objective of this study was to evaluate the compatibility of aqueous and alcoholic plant extracts, alternative products and basidiocarps of the fungus Pycnoporus sanguineus on Metarhizium anisopliae. These plants and the basidiocarps were collected and transferred to drying oven at 40°C for 7 days and then ground into a fine powder. The extracts and basidiocarps were used in 10% concentration and the alternative products in 3 concentrations, being established on product labels (CR), half (0,5CR) and twice (2CR). All treatments were sprayed on the already inoculated fungus in PDA culture medium. Viability was evaluated by direct counting of viable and unviable conidia after incubation for 16 h at 26 ± 1 º C, 12h photoperiod. For Colony Forming Unit (CFU), it was counted the number of colonies after 5 days of incubation. To vegetative growth and production of conidia, the fungus was inoculated in three points on the culture medium surface of each petri dish, staying for 7 days for subsequent colonies measurement and counting conidia. For the viability was found that all aqueous extracts, except the chinaberry differed from the control. For the CFU, only cinnamon and laurel extracts and P.sanguineus not differed from the control. In diameter, lemon grass, rue, chinaberry, cinnamon, citronella and rosemary extracts differed from the control. For conidiogeneous, lemon grass, rue, cinnamon and rosemary extracts and P. sanguineus also had significance in relation to control. All the alcoholic extracts, for viability, differed from the control. However, for CFU these same extracts showed no significance. For the diameter, it was a significant difference to the alcoholic extracts of rue, castor, neem and chinaberry. For the conidia production there were significant difference between alcoholic extracts of rue, castor, chinaberry, cinnamon, neem, rosemary and laurel. For the alternative products, in the viability parameter, all products showed significant difference in relation to control, except Forth (0,5 CR and CR) and Agro-Mos® in CR. For CFU, there was significant difference at all concentrations tested. For the diameter, only 0,5CR Pironim® differed at the control. For the conidia production, Plant Clean® (0.5 CR and CR), Pironim® (CR), Bordeaux Mixture (0.5 CR and CR) and Sulfur Mixture (CR and 2CR) showed significant differences compared to control. Despite of the biological parameters present percentage change (reduction or stimulation), there was compatibility for aqueous and alcoholic extracts. In relation to alternative products, only Sulfur Mixture was incompatible for the fungus M. anisopliae.