Construção de uma biblioteca de fragmentos de anticorpos monoclonais apresentados em fagos e seleção de clone neutralizante de toxinas presentes na peçonha de Bothrops pauloensis

• Ano de defesa: 2008
• Autor(a) principal: Cardoso, Rone
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Uberlândia; BR; Programa de Pós-graduação em Genética e Bioquímica; Ciências Biológicas; UFU
• Palavras-chave em Português: ScFv; Metaloprotease; Peçonha botrópica; Hemorragia; Genética molecular; Cobra venenosa - Veneno; Anticorpos monoclonais; Phage display; Metalloprotease; Bothropic venom; Hemorrhagyc; CNPQ::CIENCIAS BIOLOGICAS::GENETICA
• Link de acesso: https://repositorio.ufu.br/handle/123456789/15696

Metalloproteinases are hydrolytic endopeptidase-like enzymes that depend on the ligation of a metal íon, such as zinc, in the catalytic site for its activity. These enzymes are the main components of bothropic venoms that are responsible for the typical hemorrhagic effect. In this study, we have constructed a combinatorial library of scFv monoclonal antibodies derived from immunized chickens with crude venom of Bothrops pauloensis. The scFv library was screened for ligands to the toxins of the crude venom in order to identify antibodies that were able to inhibit the toxic activities caused by B. pauloensis venoms. Biological and enzymatic assays demonstrated that one scFv clone was able to inhibit the hemorrhagic and fibrinogenolytic activities of the crude venom and of an isolated metaloprotease (Neuwiedase).