Atuação da rP21 no reparo cutâneo de camundongos Balb/C

• Ano de defesa: 2018
• Autor(a) principal: Barbosa, Jeranice Silva
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Uberlândia; Brasil; Programa de Pós-graduação em Biologia Celular e Estrutural Aplicadas
• Palavras-chave em Português: Reparo; Repair; Pele; Skin; P21 recombinante; P21 recombinant; CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::CITOLOGIA E BIOLOGIA CELULAR
• Link de acesso: https://repositorio.ufu.br/handle/123456789/28880; http://doi.org/10.14393/ufu.di.2020.204

The search for new products that can optimize the repair process, or even address the inherent scar deficiencies of certain individuals, has motivated several studies and the creation of protocols to treat different types of injuries. In the present study, rP21 was tested because it has a proinflammatory profile acting in the recruitment of cells of the immune system, in the cellular cytoskeleton inducing polymerization, besides acting in angiogenesis. In order to verify the role of this protein in tissue repair, 48 male Balb / C mice were divided into four treatment groups and two control groups, after which purified rP21 was incorporated and carbopol gel was incorporated. Each lesion was treated with 1 and 50 μg of the protein for three and seven days. Then, the animals were euthanized and the skin lesion regions collected for histological and biochemical analysis. Our results demonstrated that rP21 was able to improve mast cell and macrophage recruitment improved neutrophil recruitment after three days of treatment and after seven days the activities of this last cell were decreased. Regarding angiogenesis, anti-angiogenic protein profile was observed. The results also show a greater deposition of collagen types I and III. Collagen augmentation enhances matrix resistance, promotes cell migration and proliferation, essential for wound resolution. RP21 was effective in repairing skin wounds, reducing neutrophil activity with anti-inflammatory effect and increasing total collagen deposition. Increased collagen deposition strengthens and confers matrix resistance, and promotes migration and proliferation of cells essential for wound resolution. Wound closure was assessed by daily site measurements, and it was found to improve wound closure. We include from the data obtained that the rP21 protein has therapeutic potential in tissue repair, deserving targeted studies.