Efeitos do extrato de chá verde sobre marcadores inflamatórios e lipolíticos no tecido adiposo de camundongos

• Ano de defesa: 2011
• Autor(a) principal: Cunha, Claudio Alexandre [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Adipose tissue; Cytokines; Inflammation; Lipolysis; Green tea; Citocinas; Inflamação; Lipólise; Chá verde; Tecido adiposo
• Link de acesso: http://repositorio.unifesp.br/handle/11600/9422

Objective: To analyze the effects of green tea extract on the production of cytokines and anti-inflammatory (TNF-á, IL-6, IL-10 and adiponectin) in mesenteric adipose tissue, as well as protein analysis of inflammatory signaling pathway TLR-4, MyD88, TRAF6 and lipolytic. To analyze the expression of the protein structures involved in lipolysis ATGL, ABHD5 (CGI-58), LSH and PERI in the mesenteric adipose tissue of mice treated with commercial diet and lipids and the possible changes caused by concomitant use of ecstasy green tea. Methods: Swiss mice, aged twelve weeks animals were divided into four groups: commercial diet and water gavage (CA), commercial diet green tea and gavage (CC), high-fat diet and gavage water (HA) and high-fat diet and gavage green tea. After eight weeks of treatment, animals were sacrificed. The blood was collected to obtain serum, and deposits of white adipose tissue, epididymal, retroperitoneal and mesenteric, as well as gastrocnemius skeletal muscle and liver were dissected immediately after sacrifice and analyzed. By ELISA was performed content analysis of the cytokine adiponectin, IL-6, IL-10, TNF-á. By the method of Western blotting we analyzed the contents of TLR-4 and its signaling pathway, MyD88 and TRAF6, as well as proteins involved in lipolysis ATGL, LSH, and the PERI ABHD5 (CGI-58). Results: The green tea extract increased the serum HDL cholesterol and adiponectin, as well as the concentration of IL-6, IL-10, TNF-á and the relationship IL-10/TNF-á in adipose tissue in the commercial diet. The TLR-4 and MyD88 were decreased when the fat diet was associated with green tea. The proteins involved in lipolysis ATGL, LSH, and the PERI ABHD5 (CGI-58), were increased with the consumption of green tea. Conclusion: The extract of green tea improved the serum profile of the animals treated with high-fat diet, compared to the increased concentration of HDL cholesterol and adiponectin. Millie a favored antiinflammatory, shown by an increase in the ratio IL-10/TNF-á, and decreased protein expression of TLR4 and its signaling pathway. Additionally, increased expression of proteins involved in lipolysis.