Características comportamentais, eletrofisiológicas e neuropatológicas da ocorrência de hipertensão em ratas grávidas

• Ano de defesa: 2019
• Autor(a) principal: Oliveira, Leandro Freitas [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Pre-Eclampsia; Electrophysiological Studies; Hypertension; Blood Pressure; Pré-Eclâmpsia; Estudo Eletrofisiológico; Hipertensão; Pressão Arterial
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=8000887; https://repositorio.unifesp.br/handle/11600/59966

Pre-eclampsia (PE) affects approximately 2 to 8% of pregnant women, causing blood pressure above 140 x 90 mmHg and proteinuria, normally after the 20th gestation week. If unsuccessfully treated, PE can lead to self-sustained seizures (Eclampsia) that could eventually result in death of the mother and her fetus. The present study reports an experimental model of preeclampsia hypertension in pregnant (HP) and non-pregnant (H) Wistar rats by partially clamping one of their renal arteries. Pregnant (P) and non-pregnant (C) controls were provided. Differently from controls (C and P), H and GH animals presented a steady rise in BP two weeks after renal artery clamping. Injection of pentylenetetrazol induced behavioral and electroencephalographic seizures in all groups, which were increased in number, duration, amplitude and power accompanied by decreased latency in GH animals (p<0.05). Consistent results were obtained in in vitro experimentation. Immunohistochemistry of hippocampus tissue in GH animals showed decreased density of neurons in nuclei CA1, CA3 and Hilus and increased density of astrocytes in CA1, CA3 and gyrus (p<0.05). The present findings show that the clamping of one renal arteries to 0.15mm and PTZ administration were able to induce signs similar to human PE in pregnant Wistar rats.