Caracterização fenotípica e molecular de amostras de Staphylococcus coagulase negativo isoladas de infecções da corrente sanguínea de pacientes de dois hospitais gerais da cidade de São Paulo

Detalhes bibliográficos
Ano de defesa: 2009
Autor(a) principal: Souza, Alinne Guimarães de [UNIFESP]
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: https://repositorio.unifesp.br/handle/11600/9185
Resumo: Coagulase negative staphylococci (CoNS) are important etiologic agents responsible for healthcare related infection (HCRI) in bloodstream infections. In a retrospective study we evaluate CoNS isolated from patients with HCRI in bloodstream infections admitted to two general hospitals at São Paulo city, from August 2005 to August 2007. The isolates were characterized at species level and antimicrobial susceptibility tested by the Vitek® system and ViteK® 2. Oxacillin resistance was evaluated by oxacillin and cefoxitin discs. The presence of mecA gene and the SCCmec type were determined by multiplex PCR. Staphylococcus epidermidis was the predominant specie followed by S. hominis, S. haemolyticus, S. simulans, S. warneri, S. capitis and S. auricularis. Oxacillin resistance was observed in 88.2% of the isolates with 100% concordance between oxacilin and cefoxitin discs confirmed by mecA gene. All isolates were susceptible to vancomycin and four isolates revealed intermediate resistance to teicoplanin by Etest®. One S. epidermidis showed resistance to linezolide. The predominant SCC type was the type III followed by the types IV, I, II and V. In 26.9% positive mecA isolates we did not characterize the SCCmec type by the molecular protocol utilized.