Avaliação histológica, da apoptose e da proliferação no endométrio de mulheres com síndrome dos ovários policísticos

• Ano de defesa: 2013
• Autor(a) principal: Giordano, Luiz Augusto [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: endometrium; polycystic ovary syndrome; apoptosis; proliferation; endométrio; síndrome dos ovários policísticos; apoptose; proliferação
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=855216; https://repositorio.unifesp.br/handle/11600/46735

Introduction: The Polycystic Ovary Syndrome (PCOS) is a common endocrinopathy in women and can determinate infertility and endometrial diseases. The study of endometrial proliferation and apoptosis in these patients is still unclear. Objective: Evaluate the proliferation of endometrial cells by Ki67 and endometrial apoptosis by cleaved caspase-3, Bcl-2, Fas and FasL in patients with polycystic ovary syndrome compared with control patients, with normal cycles. Patients and methods: A cross-sectional and case-control study, including patients with polycystic ovary syndrome (PCOS) diagnosed using the criteria of the Androgen Excess Society Polycystic Ovary Syndrome (AE - PCOS , 2006) . We analysed 11 patients with PCOS, called GSOP, and eight patients in the control group , named GC . We analyzed clinical data ( menstrual cycles and body mass index ), metabolic data ( fasting glucose , total cholesterol and fractions , triglycerides ) , hormonal data ( total and free testosterone , basal insulin , androstenedione , dehydroepiandrosterone sulfate , 17 - hydroxyprogesterone , thyroid-stimulating hormone , prolactin , follicle stimulating hormone , luteinizing hormone ) and morphological study of the endometrium in both groups. Morphological and immunohistochemical analysis were performed with endometrial biopsies, obtained with video - hysteroscopy. Biopsies were performed in any period of the menstrual cycle in GSOP, because all were in amenorrhea, and in the proliferative phase of the menstrual cycle in GC . Evaluation of endometrial apoptosis for each protein was performed by semiquantitative analysis. Results: The endometria of women with PCOS showed more leukocyte infiltration compared with endometrial of GC. In GSOP, we found significant decrease in immunoreactivity of cleaved caspase-3 and Ki67. No change was observed in Bcl-2, Fas and FasL. Conclusion: Women with PCOS have differences in endometrial apoptosis due to lower expression of caspase-3. The endometrial proliferation index was not increased, in relation to proliferative phase of control patients. Our data suggest that the reduction in apoptosis may be linked to the onset of endometrial diseases.