Análise da expressão gênica no epitélio endocervical de mulheres com endometriose profunda

• Ano de defesa: 2015
• Autor(a) principal: Kopelman, Alexander [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: endometriosis; endometriose
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3197874; https://repositorio.unifesp.br/handle/11600/46730

SUMMARY: The lack of diagnostic markers of endometriosis causes the surgery to be performed for this purpose. It is an invasive method of high cost contributing to the global scenario, where there has been an average of seven years before the disease is diagnosed from the first symptoms. Objective: This study is based on the changes of gene expression in the endometrium of women with endometriosis. Since the body and cervix have common embryonic origin and receptors for estrogen and progesterone have forum described in the cervix, this study aims to identify whether there are differences in gene expression in endocervical epithelium with the prospect of creating a non-invasive test for the diagnosis of disease. Methods: We performed RT-PCR analysis of the endocervical epithelium of 4 women with deep endometriosis and 6 healthy women. RESULTS: Eight genes showed overexpression in samples of women with endometriosis, five genes of genes related to cell cycle control (CCNB1, CCNG1, CUL1, GTF2H1, PCNA) and three genes inflammatory cytokines (C3, CCL21 and CXCL14). CONCLUSION: Analysis of gene expression in endocervical epithelium of women with endometriosis is a promising non-invasive screening tool for the diagnosis of endometriosis