Efeitos da glicina nas expressões imunohistoquimicas de PDGF, FGF e EGF em mucosite oral induzita em hamster

• Ano de defesa: 2017
• Autor(a) principal: Sá, Odara Maria de Sousa [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Stomatitis; Oral mucositis; Glycine; Intercellular signaling peptides and proteins; Growth factors; Collagen; Estomatite; Mucosite oral; Glicina; Peptídeos e proteínas de sinalização intracelular; Fatores de crescimento; Colágeno
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5570446; http://repositorio.unifesp.br/handle/11600/50377

Introduction: Oral mucositis is one of the most frequent toxic effects of chemotherapeutic and/or radiotherapeutic treatment, resulting from complex multifaceted biological events involving DNA damage, multiple signaling, and interactions between epithelial, submucosal, and connective tissue. Clinical manifestations such as pain, difficulty in swallowing and communication, and infections have a negative impact on the tolerance to treatment and the quality of life of cancer patients. Preventive measures and curative treatment of mucositis, secondary to chemotherapy and radiotherapy, are still not well established, requiring cooperative studies and new synchronic and effective therapeutic approaches. On the other hand, the nonessential amino acid glycine has anti-inflammatory, immunomodulatory and cytoprotective action, being a potential therapeutic option in mucositis. Therefore, we performed this study with the objective of evaluating the effects of glycine on the expression of collagen and growth factors, platelet (PDGF) and epidermal (EGF), in experimental models of oral mucositis, as apoptotic markers. Methods: The biological material used was the hamster jugal mucosa. The mucositis of which was induced by the protocol proposed by Sonis. A total of 40 hamsters were used, divided into two groups: group I (n= 20)- control; Group II (n= 20) supplemented with 5% intraperitoneal glycine, 0,2 ml (2,0 mg /g) diluted in herpes . On day 7 histopathological sections were performed in order to perform the immune-histochemical method, the evaluation of quantitative and qualitative collagen expression by means of picrossirius under polarized light and the growth factors: EGF and PDGF. Results: It was observed that the group supplemented with glycine higher amounts of collagen expression and predominance type of collagen I when compared to the compared to the control group (p≤0.0002). The glycine group presented lower immunoexpression (p≤0,0001) of the growth factors, EGF and PDGF in relation to the control group. The animals that expressed type I collagen fibers, independent of the experimental group, presented higher amount of collagen and less immunoexpression of the growth factors: EGF and PDGF. Conclusion: The group supplemented with glycine showed a marked healing process of the oral mucosite, demonstrated by the predominance of collagen type I and reduction of growth factors, EGF and PDGF.