Fatores pré-analíticos determinantes no valor diagnóstico do exame citopatológico em cães e gatos

• Ano de defesa: 2024
• Autor(a) principal: Moraes, Bibiana Teló
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Santa Maria; Brasil; Medicina Veterinária; UFSM; Programa de Pós-Graduação em Medicina Veterinária; Centro de Ciências Rurais
• Palavras-chave em Português: Citopatologia; Celularidade; Rompimento celular; Contaminação sanguínea; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Link de acesso: http://repositorio.ufsm.br/handle/1/33801

In veterinary medicine, cytopathology is considered a low-invasive, easy-to-collect and low-cost diagnostic screening technique. One of the main determining factors in the diagnostic value of this technique is the quality of the sample. This study’s goal was to identify the main factors that determined representative and nonrepresentative diagnoses of cytopathological samples from dogs and cats. Sample analysis was carried out in the clinical analysis lab QUALEM- Veterinary Laboratory, in the city of Santa Maria, Rio Grande do Sul. The samples came from routine lab work, received from clinics in the central region. The criteria evaluated in the samples were cellularity, cell disruption and blood contamination. Statistical analyzes were evaluated descriptively and subsequently submitted to the Mann-Whitney t-test for non-parametric samples, which is significant when p ≤ 0.05. Regarding cellularity, this was significantly (p<0.01) higher in the group of representative samples, totaling 236 samples (63.5%) with a high number of cells and 50 samples (47.2%) showed an absence of diagnostic cellularity in the non-representative samples group. Cell disruption was significantly (p<0.01) greater in nonrepresentative slides, which resulted in 27 (25.4%) samples with a high degree of cell disruption. It was observed that these samples came from material collected from lymph nodes and the technique of choice was FNAC (18 – 17.0%). In addition, a greater (p<0.01) blood contamination was observed in slides with a non-representative cytopathological diagnosis, resulting in 60 samples (56.6%) with this criterion evaluated. Samples with representative diagnosis were segregated into groups of neoplastic (n=180) and non-neoplastic (n=190) lesions. Respectively to the groups, there was a prevalence of lipoma (42 – 23.3%), mast cell tumor (26 – 14.4%) and lymphoma (18 – 8.9%); and septic suppurative inflammation (23 – 12.1%), pyogranulomatous inflammation (18 – 9.5%) and hyperplastic/reactive lymph node (17 – 8.9%). From the results obtained, it was evident that success in obtaining representative samples is associated with the professional's experience, the collection technique used and the correct handling and preparation of samples.