Marcadores associados a características reprodutivas de touros

• Ano de defesa: 2017
• Autor(a) principal: Ferreira, Carlos Eduardo Ranquetat
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Pelotas; Faculdade de Veterinária; Programa de Pós-Graduação em Veterinária; UFPel; Brasil
• Palavras-chave em Português: Exame andrológico; Fertilidade; Paraoxonase; Perímetro escrotal; Seleção genômica; Breeding soundness evaluations; Fertility; Genomic selection; Scrotal perimeter; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL
• Link de acesso: http://guaiaca.ufpel.edu.br/handle/prefix/3956

Male fertility is important to optimize pregnancy rates and genetic improvement. Breeding soundness evaluations are key tools to identify sub fertile males and to survey characteristics of sperm quality. However, few sperm quality traits are correlated with fertility in vivo. Thus, it is essential to identify methods that can assist in more precise selection of future bulls. The goals of the first study were to evaluate the accuracy and the bias of the genomic predictions, using different methods, for the characteristic scrotal perimeter adjusted to age and adjusted to age and weight in Hereford and Braford bulls. The estimates for the heritability showed moderate to high magnitude (0.39 to 0.48), demonstrating that it is possible to obtain genetic gains from selection. The prediction of genetic values using genomic information by methods of selection index and single-step made possible the increase of accuracy (around 30%) for the characteristics studied. The gains in accuracy obtained with the methods, combining the traditional with the genomic information compared to traditional BLUP method indicate that the genomic predictions can be used as a tool to improve the genetic gains and reduce the range of generations. The second article had as its objectives: to determine the mRNA expression of paraoxonases (PON) 1, 2 and 3 in the testicular parenchyma, seminal vesicles, and epididymis of bulls, to evaluate the activity of PON1 in the bloodstream and in seminal plasma; and to correlate that activity with characteristics of breeding soundness. Characterization of gene expression by qRT-PCR and the determination of PON1 activity were performed in serum and seminal plasma from bulls 110. The PON 1, 2 and 3 were expressed in testicular parenchyma of the animals examined. There was a positive correlation between activity and serum PON1 seminal with different breeding soundness estimators.